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X-Ray microanalysis of the secretory granules in goblet cells of mouse intestinal tracts: Changes with age

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Abstract

Changes in the elements in goblet cell secretory granules in three portions of the intestinal tract — duodenum, proximal colon, and distal colon — in mice from 14 days to 18 months after birth were studied by quantitative electron probe X-ray microanalysis on quick-frozen and freeze-dried cryosections by calculating the ratio of each element. In the analysis of five elements, the peaks decreased in the order of sulfur (S), potassium (K), chlorine (Cl), phosphorus (P), and calcium (Ca) in the duodenum at 1 month; in the proximal colon, the peaks declined in the order of K, S, Cl, Ca, and P at 3 months; and in the distal colon, the peaks declined as S, K, Ca, Cl, and P at 3 months. The highest average ratio of S was obtained in the duodenum at 1 month, and this value then declined with age. In the proximal colon, the average ratio of K was the highest at 3 months, then declined afterward with age. The highest average ratio of S was at 3 months, and the highest average ratio of K was at 1 month in the distal colon. The ratio of peak counts to the background was calculated for each type of granule. Significant differences were found in the ratios of S and K between the proximal and distal colon. As for S, the duodenum and the distal colon had the highest ratios and the proximal colon had the second highest. Concerning K, the proximal colon had the highest and the duodenum and the distal colon the second highest ratio. The secretory granules of goblet cells in the three portions of the intestinal tract were shown to contain different amounts of each element. Especially, the amount of S differed most from the proximal to the distal colon.

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References

  1. Strous GJ, Dekker J (1992) Mucin type glycoprotein. Crit Rev Biochem Mol Biol 27:57–92

    Google Scholar 

  2. Spicer SS, Schulte BA (1992) Diversity of cell glycoconjugates shown histochemically: a perspective. J Histochem Cytochem 40:1–38

    Google Scholar 

  3. Murata F, Tsuyama S, Ihiida K, Kashino N, Kawano M, Zeug ZL (1992) Sulfated glycoconjugates demonstrated in combination with high iron diamine tiocarbohydrazide-silver proteinate and silver acetate physical development. J Electron Microsc 41:14–20

    Google Scholar 

  4. Sheahan DG, Jervis HR (1976) Comparative histochemistry of gastrointestinal mucosubstance. Am J Anat 146:103–132

    Google Scholar 

  5. Park CM, Reid PE, Owen DA, Volz D, Dunn WL (1987) Histochemical studies of epithelial cell glycoproteins in normal rat colon. Histochem J 19:546–554

    Google Scholar 

  6. Nagata T (1991) Electron microscopic radioautography and analytical electron miscroscopy. J Clin Electron Microsc 24:441–442

    Google Scholar 

  7. Maruyama M (1991) Histochemical studies on glycoprotein synthesis in the colonic goblet cells of mice (in Japanese). Shinshu Med J 39:467–478

    Google Scholar 

  8. Maruyama M, Nagata T (1987) X-ray microanalysis with a high voltage electron microscope quantifying sulfur in colonic goblet cells of aging mice. J Clin Electron Microsc 20:678–679

    Google Scholar 

  9. Ichikawa R, Hayashi K, Nagata T (1994) X-ray microanalysis of the secretory granules in the intestinal goblet cells of aging mice. Med Electron Microsc 27:337–339

    Google Scholar 

  10. Nagata T, Maruyama M, Ichikawa R, Usuda N (1988) X-ray microanalysis of biological specimens by high voltage transmission electron microscopy. In: Proceedings, 8th internat congress of histochemistry and cytochemistry, Washington, D.C. J Histochem Cytochem 36:858–858

  11. Ono S (1991) Electron microscopy and electron probe X-ray microanalysis of human lumbar yellow ligaments by frozen-dried cryosections. J Clin Electron Microsc 24:377–388

    Google Scholar 

  12. Mizuhira V, Shiihasi M (1978) Quantitative X-ray microanalysis of a mouse cardiac muscle by means of the freeze substitution method. Proc. 9th Int Cong Electron Microsc Toronto II:124–125

    Google Scholar 

  13. Mizuhira V (1979) Elemental analysis of biological specimens by electron probe X-ray microanalysis. In: Tsuruta TF (ed) Chemistry for the welfare of mankind. Proceedings of the 26th international congress of pure and applied chemistry. Tokyo, 1977, vol 26. Pergamon Press Oxford, pp A1-A19

    Google Scholar 

  14. Takaya K (1984) Energy dispersive X0ray microanalysis of platelets and megakaryocytes of several mammals using air-dried spreads and fresh frozen dried sections. J Phys Colloq C 45 (suppl 2):C2-485–C2-489

    Google Scholar 

  15. Merzel J, Leblond CP (1969) Origin and renewal of goblet cells in the epithelium of the mouse small intestine. Am J Anat 124:281–306

    Google Scholar 

  16. Altmann GG (1983) Morphological observations on mucus-secreting nongoblet cells in the deep crypts of the rat ascending colon. Am J Anat 167:95–117

    Google Scholar 

  17. Kawahara I (1996) Histochemical studies on sulfomucin synthesis in the digestive tracts of mice (in Japanese). Shinshu Med J 44:17–36

    Google Scholar 

  18. Durnam DM, Palmiter RD (1981) Transcriptional regulation of the mouse metallothionein-l gene by heavy metals. J Biol Chem 256:5712–5716

    Google Scholar 

  19. Gupta BL, Hall TA, Naftalin RJ (1978) Microprobe measurement of Na, K, and Cl concentration profiles in epithelial cells and intercellular spaces of rabbit ileum. Nature (Lond) 272:71–73

    Google Scholar 

  20. Hale G, Streeter HB, Hann AC, Gacesa P, Wusteman FS (1987) Chondroitin sulphate at the endothelial lumen of pig aorta: assay by radiolabelling and X-ray microanalysis. Biosci Rep 7:187–191

    Google Scholar 

  21. Modis L, Lustyik G, Adany R, Nazy IZ (1988) Energy dispersive X-ray microanalysis of sulfated glycosaminoglycans in cartilage matrix stained with alcian blue 8GX. Basic Appl Histochem 32:415–428

    Google Scholar 

  22. Reid PE, Cullings CFA, Dunn WL, Ramey CW, Clay MG (1984) Chemical and histochemical studies of normal and diseased human gastrointestinal tract. I. A compositon between histologically normal colon, colonic tumours, ulcerative colitis and diverticular disease of the colon. Histochem J 16:235–251

    Google Scholar 

  23. Reid PE, Culling CF, Dunn WL, Clay MG (1984) Chemical and histochemical studies of normal and diseased human gatrointestinal tract. II. A comparison between histologically normal small intestine and Crohn's disease of the small intestine. Histochem J 16:253–264

    Google Scholar 

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Authors and Affiliations

  1. General Research Laboratory, Shinshu University School of Medicine, 3-1-1 Asahi, 390-8621, Matsumoto, Japan

    Kiyokazu Kametani & Ryouji Ichikawa

  2. Department of Anatomy and Cell Biology, Shinshu University School of Medicine, Matsumoto, Japan

    Tetsuji Nagata

Authors
  1. Kiyokazu Kametani
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  2. Ryouji Ichikawa
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  3. Tetsuji Nagata
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Kametani, K., Ichikawa, R. & Nagata, T. X-Ray microanalysis of the secretory granules in goblet cells of mouse intestinal tracts: Changes with age. Med Electron Microsc 31, 107–114 (1998). https://doi.org/10.1007/BF01557788

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  • DOI: https://doi.org/10.1007/BF01557788

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