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A procedure for the fusion of cells in suspension by means of polyethylene glycol

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  • Published:
Somatic Cell Genetics

Abstract

The nuclei of chick and human fibroblasts are readily distinguishable in Leishman stained heterokaryons. The use of these two types of cell has allowed development of a method for fusion in suspension based on the use of polyethylene glycol (PEG) of MW 1000 in Dulbecco's medium containing 15% dimethylsulfoxide (DMSO).

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Authors and Affiliations

  1. Imperial Cancer Research Fund, Lincoln's Inn Fields, P.O. Box 123, WC2A 3PX, London, England

    Anne Hales

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  1. Anne Hales
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Hales, A. A procedure for the fusion of cells in suspension by means of polyethylene glycol. Somat Cell Mol Genet 3, 227–230 (1977). https://doi.org/10.1007/BF01551817

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  • DOI: https://doi.org/10.1007/BF01551817

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