Abstract
Somatic cell hybrids between hepatoma and Friend erythroleukemia parental cells were studied for the expression of liver-specific and erythroid properties. Several independent clones were isolated using HAT selection and were shown to be true hybrids by isozyme and chromosome analysis. All displayed a complete extinction of hemoglobin and globin mRNA production, but a retention of albumin and transferrin secretion. The data suggest that erythroid differentiation is being actively inhibited by the hepatoma genome. Possible mechanisms that might explain these results are discussed in the light of current hypotheses regarding the mechanism of cell differentiation.
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Literature cited
Davidson, R.L. (1973). InGenetic Mechanisms of Development, Ruddle, F.H. (ed.), Academic Press, New York, pp. 295–327.
Darlington, G.J., and Ruddle, F.H. (1975). InModern Trends in Human Genetics, Emery, A.E.H. (ed.), Vol. 2, Butterworth and Co., London, pp. 111–137.
Deisseroth, A., Burk, R., Picciano, D., Minna, J., Anderson, W.F., and Nienhuis, A. (1975).Proc. Natl. Acad. Sci. USA 72:1102–1106.
Orkin, S.H., Harosi, F.I., and Leder, P. (1975).Proc. Natl. Acad. Sci. USA 72:98–104.
Skoultchi, A., Conscience, J.-F., and Ruddle, F.H. Manuscript in preparation.
Friend, C., Preisler, H.D., and Scher, W. (1974).Curr. Top. Dev. Biol. 8:81–101.
Conkie, D., Affara, N., Harrison, P.R., Paul, Jr., and Jones K. (1974).J. Cell Biol. 63:414–419.
Stern, R., Boyer, S., Conscience, J.-F., Margolet, L., Tashian, R., and Ruddle, F.H. Manuscript in preparation.
Conscience, J.-F., Miller, R., Henry, J., and Ruddle, F.H. Manuscript in prepatation.
Friend, C., Scher, W., Holland, J.G., and Sato, T. (1971).Proc. Natl. Acad. Sci. USA 68:378–382.
Darlington, G.J., Bernhard, H.P., and Ruddle, F.H. (1974).Science 185:859–862.
Scher, W., Holland, J.G., and Friend, C. (1971).Blood 37:428–437.
Littlefield, J.W. (1964).Science 145:709–710.
Giles, R.E., and Ruddle, F.H. (1973).In Vitro 9:108–107.
Puck, T.T., Marcus, P., and Ciecura, S.J. (1956).J. Exp. Med. 103:273–284.
Nichols, E.A., and Ruddle, F.H. (1973).J. Histochem. Cytochem. 21:1066–1081.
Clausen, J. (1969).Immunochemical Techniques for the Identification and Estimation of Macromolecules, North Holland Publishing Company, Amsterdam.
Aviv, H., and Leder, P. (1972).Proc. Natl. Acad. Sci. USA 69:1408–1412.
Bernhard, H.-P., Darlington, G.J., and Ruddle, F.H. (1973).Develop. Biol. 35:83–96.
Sumner, A.T., Evans, H.J., and Buckland, R.A. (1971).Nature New Biol. 232:31–32.
Houseman, D., Skoultchi, A., Forget, B.G., and Benz, E.J. Jr. (1974).Ann. N.Y. Acad. Sci. 241:280–289.
Hrinda, M.E., and Goldwasser, E. (1969).Biochim. Biophys. Acta 195:165–175.
Deisseroth, A., Velez, R., Burk, R.D., Minna, J., Anderson, W.F., and Nienhuis, A. (1976).Somatic Cell Genet. 2:373–384.
Paul, J., and Hickey, I. (1974).Exp. Cell Res. 87:20–30.
Gopalakrishnan, T.V., and Thompson, E. Brad (1976).J. Cell Biol. 70:51a.
Szpirer, J., and Szpirer, C. (1975).Cell 6:53–60.
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Conscience, J.F., Ruddle, F.H., Skoultchi, A. et al. Somatic cell hybrids between Friend erythroleukemia cells and mouse hepatoma cells. Somat Cell Mol Genet 3, 157–172 (1977). https://doi.org/10.1007/BF01551812
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DOI: https://doi.org/10.1007/BF01551812