Abstract
Nonsynchronized and hydroxyurea (HU)-synchronized SV40-transformed human cells (W98VaD) were fused with chick embryo erythrocytes (CE). The uptake of T antigen by CE nuclei was compared with initiation of chick nuclear DNA synthesis. Uptake of T antigen by CE nuclei occurred at about the same timeafter fusion with asynchronous as with HU-synchronized cells. CE nuclei rapidly became T antigen-positive between 16 h and 28 h after fusion and usually almost all CE nuclei were T antigen-positive by 48 h after fusion. In contrast, initiation of chick nuclear DNA synthesis occurred as a function of timeafter reversal of the HU block, when the host cell nuclei were also synthesizing DNA. Chick nuclear DNA synthesis occurred in many heterokaryons before the CE nuclei became T antigen-positive by immunofluorescence.
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Tegtmeyer, P. (1972).J. Virol. 10:591–598.
Reed, S.I., Ferguson, J., Davis, R.W., and Start, G.R. (1975).Proc. Natl. Acad. Sci. USA 72:1605–1609.
Tenen, D.G., Baygell, P., and Livingston, D.M. (1975).Proc. Natl. Acad. Sci. USA 72:4351–4355.
Carroll, R.B., Hager, L., and Dulbecco, R. (1974).Proc. Natl. Acad. Sci. USA 71:3754–3757.
Kit, S., Dubbs, D.R., Piekarski, L.J., deTorres, R.A., and Melnick, J.L. (1966).Proc. Natl. Acad. Sci. USA 56:463–470.
Kit, S., Dubbs, D.R., Frearson, P.M. (1966).Virology 29:69–83.
Martin, R.O., and Chou, J.Y. (1975).J. Virol. 15:599–612.
Tegtmeyer, P. (1975).J. Virol. 15:613–618.
Brugge, J.S., and Butel, J.S. (1975).J. Virol. 15:619–635.
Osborn, M., and Weber, K. (1975).J. Virol. 15:636–644.
Cameron, I.L., and Prescott, D. (1963).Exp. Cell Res. 30:609–612.
Johnson, R.T., and Harris, H. (1969).J. Cell Sci. 5:625–643.
Dubbs, D.R., and Kit, S. (1976).Somat. Cell Genet. 2:11–19.
Harris, H. (1974).Nucleus and Cytoplasm, 3rd Ed., Clarendon Press, Oxford, pp. 109–141.
Ringertz, N.R., Carlsson, S.-A., Ege, T., and Bolund, L. (1971).Proc. Natl. Acad. Sci. USA 68:3228–3232.
Appels, R., Bolund, L., Goto, S., and Ringertz, N.R. (1974).Exp. Cell Res. 85:182–190.
Rosenqvist, M., Stenman, S., and Ringertz, N.R. (1975).Exp. Cell Res. 92:515–518.
Girardi, A.J., Jensen, F.C., and Koprowski, H. (1965).J. Cell. Comp. Physiol. 65:69–84.
Kit, S., Kurimura, T., Brown, M., and Dubbs, D.R. (1970).J. Virol. 6:69–77.
Kit, S., and Dubbs, D.R. (1962).Virology 18:274–285.
Bolund, L.N., Ringertz, N.R., and Harris, H. (1969).J. Cell Sci. 4:71–87.
Kit, S., Dubbs, D.R., and Hsu, T.C. (1963).Virology 19:13–22.
Kaplan, M.M., Giard, D.J., Blattner, W.A., Lubiniecki, A.S., and Fraumeni, J.F. (1975).Proc. Soc. Exp. Biol. Med. 148:660–664.
Steplewski, Z., Knowles, B.B., and Koprowski, H. (1968).Proc. Natl. Acad. Sci. USA 59:769–776.
Pardee, A.B. (1974).Proc. Natl. Acad. Sci. USA 71:1286–1290.
Pardee, A.B., and James, L.G. (1975).Proc. Natl. Acad. Sci. USA 72:4994–4998.
Vogel, A., and Pollack, R. (1975).J. Cell Physiol. 85:151–162.
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Dubbs, D.R., Kit, S. Initiation of DNA synthesis and uptake of T antigen by chick erythrocyte nuclei in heterokaryons with SV40-transformed human cells. Somat Cell Mol Genet 3, 61–69 (1977). https://doi.org/10.1007/BF01550987
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DOI: https://doi.org/10.1007/BF01550987