Riassunto
Gli AA. riferiscono un gruppo di esperimentiin vitro riguardanti l'inibizione diretta dell'effetto dell'insulina sul trasporto attivo del 3-o-metil glucosio e della l-prolina nel diaframma di ratto da parte di un reagente specifico per il triptofano in soluzione neutra, il 2-idrossi-5-nitrobenzil bromuro. Viene così provata la presenza di triptofano in un recettore insulinico della membrana plasmatica.
Resume
Les AA. rapportent un groupe d'épreuvesin vitro au sujet de l'inhibition directe de l'effet de l'insuline sur le transport actif des 3-o-metil glucose et de la l-proline dans le diaphragme de rat par un réactif spécifique pour le triptophane en solution neutre, le 2-idrossi-5-nitrobenzil bromure. On prouve ainsi la présence de triptophane dans un récepteur insulinique de la membrane plasmatique.
Resumen
Los AA. describen un grupo de experimentosin vitro concernientes a la inhibición directa del efecto de la insulina en el transporte activo de la 3-0-metil glucosa y de la l-prolina en el diafragma de rata por parte de un reactivo específico para el triptófano en solución neutra, el 2-hidroxi-5-nitrobenzil bromuro. De esta manera se comprueba la presencia de triptófano en un receptor insulínico de la membrana plasmática.
Zusammenfassung
Die AA. berichten ueber eine Serie vonin vitro Untersuchungen betr. die direkte Hemmung des Insulineffektes auf den aktiven Transport der 3-o-methyl-Glukose und des l-Prolins beim Rattenzwerchfell mittels eines spezifischen Reagenz' fuer das Tryptophan in neutraler Loesung, des 2-hydroxy-5-nitrobenzil-bromid. Somit ist die Gegenwart von Tryptophan in einem Insulinrezeptor der Plasmamembran bewiesen.
Summary
The AA. report a group ofin vitro experiments relative to the direct inhibition of the insulin effect on the transport of 3-o-methyl glucose and L-proline in rat diaphragm muscle by a reagent specific for tryptophan in a neutral solution, the 2-hydroxy-5-nitrobenzyl bromide. Evidence for the presence of tryptophan at an insulin receptor site in the plasma membrane is given.
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Maturo, J.M., Rieser, P. Insulin receptors and membrane transport in muscle. Acta diabet. lat 5, 31–39 (1968). https://doi.org/10.1007/BF01548364
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DOI: https://doi.org/10.1007/BF01548364