Abstract
The superficial colonic epithelia of rats were exposed to 1.0% saponin solution for 3 min and fixed at various periods thereafter. The repair or restitution process was observed by light as well as by transmission and scanning electron microscopy. The exposure of the luminal surface to saponin resulted in uniform and extensive damage to the superficial epithelial cells without affecting the cells in the crypts. At 3 min after saponin treatment, the damaged epithelial cells exfoliated from the mucosa and the basal lamina was exposed. Within 15 min, most of the exposed basal lamina was covered by squamous to low-cuboidal epithelial cells, probably migrating from the crypts. These epithelial cells extended large lamellipodia over the denuded basal lamina. After 15 min the damaged surface was completely covered with epithelial cells, which became columnar at 1 h. Tight junction protein ZO-1 became positive along the restituted epithelium. Proliferating-cell nuclear antigen (PCNA) staining showed that proliferation of epithelial cells occurred after the restitution. These results suggest that saponin treatment serves as a good model system to study colonic restitution, which is carried out by rapid migration from the remaining crypt cells, followed by cellular proliferation. Rapid formation of tight junctions spanning the damaged regions allows rapid restoration of the barrier function of the colonic epithelium.
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References
Silen W, Ito S (1985) Mechanism for rapid re-epithelialization of the gastric mucosal surface. Annu Rev Physiol 47:217–229
Lacy ER, Ito S (1984) Rapid epithelial restitution of the rat gastric mucosa after ethanol injury. Lab Invest 51:573–583
Allen A, Flemström G, Garner A, Kivilaakso E (1993) Gastroduodenal mucosal protection. Physiol Rev 73:823–857
Svanes K, Ito S, Takeuchi K, Silen W (1982) Restitution of the surface epithelium of the in vitro frog gastric mucosa after damage with hyperosmolar sodium chloride: morphologic and physiologic characteristics. Gastroenterology 82:1409–1426
Ito S, Lacy ER (1985) Morphology of rat gastric mucosal damage, defense and restitution in the presence of luminal ethanol. Gastroenterology 88:250–260
Chadwick VS, Gaginella TS, Carlson GL, Debongnie JC, Phillips SF, Hofmann AF (1979) Effect of molecular structure on bile acid-induced alterations in absorptive function, permeability, and morphology in the perfused rabbit colon. J Lab Clin Med 94:661–674
Watt J, Marcus R (1971) Carrageenan-induced ulceration of the large intestine in the guinea pig. Gut 12:164–171
Wallace JL, Whittle BJR, Boughton-Smith NK (1985) Prostaglandin protection of rat colonic mucosa from damage induced by ethanol. Dig Dis Sci 30:866–876
MacPherson BR, Pfeiffer CJ (1978) Experimental production of diffuse colitis in ysts. Digestion 17:135–150
Buck RC (1986) Ultrastructural features of rectal epithelium of the mouse during the early phases of migration to repair a defect. Virchows Arch B Cell Pathol 51:331–340
Argenzio RA, Henrikson CK, Liacos JA (1988) Restitution of barrier and transport function of porcine colon after acute mucosal injury. Am J Physiol 255:G62-G71
Henrikson CK, Argenzio RA, Liacos JA, Khosla J (1989) Morphologic and functional effects of bile salt on the porcine colon during injury and repair. Lab Invest 60:72–87
Prasad M, Ito S, Silen W (1997) Functional studies of in vitro rat distal colon before and after restitution. Surgery 121:430–439
Feil W, Lacy E, Wong YM, Burger D, Wenzl E, Starlinger M, Schiessel R (1989) Rapid epithelial restitution of human and rabbit colonic mucosa. Gastroenterology 97:685–701
Stevenson BR, Siliciano JD, Mooseker MS, Goodenough DA (1986) Identification of ZO-1: a high molecular weight polypeptide associated with the tight junction (zonula occludens) in a variety of epithelia. J Cell Biol 103:755–766
Diebold J, Lai MD, Löhrs U (1992) Analysis of proliferative activity in colorectal mucosa by immunohistochemical detection of proiferating cell nuclear antigen (PCNA). Virchows Arch B Cell Pathol 62:283–289
Ogihara H, Saito H, Shin B, Terada T, Takenoshita S, Nagamachi Y, Inui K, Takata K (1996) Immuno-localization of H+/peptide cotransporter in rat digestive tract. Biochem Biophys Res Commun 220:848–852
Kirsner JB, Shorter RG (1982) Recent developments in “nonspecific” inflammatory bowel disease. N Engl J Med 306:775–785
Eastwood GL (1977) Gastrointestinal epithelial renewal. Gastroenterology 72:962–975
Sollmann T (1964) A manual of pharmacology and it applications to therapeutics and toxicology. 8th edn. Saunders, Philadelphia, pp 667–670
Gee JM, Johnson IT (1988) Interactions between hemolytic saponins, bile salts and small intestinal mucosa in the rat. J Nutr 118:1391–1397
Feil W, Wenzl E, Vattay P, Starlinger M, Sogukoglu T, Schiessel R (1987) Repair of rabbit duodenal mucosa after acid injury in vivo and in vitro. Gastroenterology 92:1973–1986
Rutten MJ, Ito S (1983) Morphology and electrophysiology of guinea pig gastric mucosal repair in vitro. Am J Physiol 244:G171-G182
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Takahashi, M., Ogihara, H., Nomingerel, T. et al. Repair of the surface epithelium after saponin-induced colonic mucosal injury in the rat. Med Electron Microsc 31, 1–9 (1998). https://doi.org/10.1007/BF01547942
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DOI: https://doi.org/10.1007/BF01547942