Skip to main content
SpringerLink
Log in

Studio del metabolismo glicidico nel tessuto adiposo e nel muscolo in rapporto all'azione di un agente chelante, la desferrioxamina

  • Lavori Originali
  • Published:
Acta diabetologia latina Aims and scope Submit manuscript

Riassunto

E' stato studiato il metabolismo del glucosio e del fruttosio nel tessuto adiposo epididimale e nel diaframma di ratto in presenza di desferrioxamina (DFOM). La DFOM aumenta il consumo di glucosio da parte di questi tessuti ed inoltre aumenta l'ossidazione e l'incorporazione negli acidi grassi del carbonio del glucosio da parte del tessuto adiposo.

Non è stato possibile dimostrare un effetto significativo della DFOM sull'utilizzazione del fruttosio da parte del tessuto adiposo epididimale del ratto.

Viene prospettata l'ipotesi che l'azione della DFOM si manifesti per effetto della sua capacità chelante, consistente nel rimuovere i ferro ioni dalla membrana cellulare o dall'interno della cellula.

A conferma di ciò sono i risultati ottenuti con la ferrioxamina, un derivato della DFOM, privo di capacità chelante perchè già saturo con ioni ferro, che ha dimostrato di non provocare alcuna modificazione sul metabolismo del glucosio nel tessuto adiposo.

Resume

Les AA. ont étudié le métabolisme du glucose et du fructose dans le gras épididymal du rat en présence de desferrioxamina (DFOM). La DFOM augmente la consume du glucose par ce tissue et augmente en plus par le tissue adipeux l'oxidation et l'incorporation dans les acides gras du carbone du glucose.

Les AA. n'ont pas eu la possibilité de démontrer un effect significatif de la DFOM sur l'utilisation du fructose par le gras épididymal du rat.

On releve l'hypothèse que l'action de la DFOM peut se développer par sa proprieté chélante, c'est à dire la possibilité de enlever les ions du fer de la membrane cellulaire ou de l'interieur de la cellule.

Ces résultats ont été confirmé par ce qui est arrivé avec la ferrioxamina, un derivé de la DFOM, sans proprieté chélante parce qu'elle est dejà saturée avec ions-fer, qui a demontré de ne porter aucune modification au métabolisme du glucose dans le tissue adipeux.

Resumen

Se ha estudiado el metabolismo de la glucosa y fructosa en el tejido adiposo epididimal y en el diafragma de rata en presencia de desferrioxamina (DFOM). La DFOM aumenta el consumo de glucosa de parte de estos tejidos y además aumenta la oxidación y la incorporación en los ácidos grasos del carbono de la glucosa de parte del tejido adiposo.

Ne se pudo demostrar un efecto significativo de la DFOM sobre la utilización de la fructosa de parte del tejido adiposo epididimal de la rata.

Se expresa la hipótesis que la acción de la DFOM se manifeste por efecto de su capacidad quelante, consistente en desplazar los hierro iones de la membrana celular o del interior de la célula.

Hay para confirmarlo los resultados obtenidos con la ferrioxamina, un derivado de la DFOM, que falta de capacidad quelante por ser ya sáturo con iones hierro y que comprobó no provocar alguna modificación sobre el metabolismo de la glucosa en el tejido adiposo.

Zusammenfassung

Es wurde der Stoffwechsel der Glukose und Fruktose im epididymalen Fettgewebe sowie im Rattenzwerchfell in Anwesenheit von Desferrioxamin (DFOM) untersucht. Das DFOM erhoeht den Glukoseverbrauch seitens dieser Gewebe und steigert ferner die Oxydation und Einverleibung des Glukosekohlenstoffs in die Fettsaeuren seitens des Fettgewebes.

Es war unmoeglich, einen signifikanten Effekt des DFOM auf die Fruktoseverwertung seitens des epididymalen Rattenfettgewebes nachzuweisen.

Es wird die Hypothese aufgestellt, dass sich die Wirkung des DFOM durch seine chelierende Faehigkeit aeussert, die darin besteht, dass die Eisenjone aus der Zellmembran oder dem inneren der Zelle entfernt werden.

Diese Daten werden durch die mit Ferrioxamin erzielten Resultate bestaetigt, einem DFOM-Derivat ohne chelierende Wirkung, da schon mit Eisenjonen gesaettigt; dieses verursacht keine Veraenderung des Glukosestoffwechsels im Fettgewebe.

Summary

Glucose and fructose metabolism in rat adipose tissue and rat diaphragm in presence of desferrioxamine (DFOM) was studied. DFOM increases glucose consumption by these tissues and glucose oxidation and glucose carbon incorporation into fatty acids by adipose tissue.

No significant effect on fructose utilization by adipose tissue was detected.

It is suggested that DFOM might be active by removal of Fe-ions from cell membrane or from cell inside, due to its chelating capacity.

This hypothesis is strenghtened by the fact that ferrioxamine (FOM), which is the chemical analogue of DFOM deprived of chelating activity, does not affect glucose metabolism in adipose and muscolar tissue.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Bibliografia

  1. Bickel H., Gaumann E., Keller-Schierlein W., Prelog V., Fischer E., Wettstein A., Zahner H.: Über eisenhaltige Wachstumsfaktoren, die Sideramine, und ihre Antagonisten, die eisenhaltigen Antibiotika Sideromycine - Experientia16 129, 1960.

    PubMed  Google Scholar 

  2. Bickel H., Hall G. E., Keller-Schierlein W., Prelog V., Fischer E., Wettstein A.: Stoffwechselprodukte von Actinomyceten 27. Mitteilung. Über die Konstitution von Ferrioxamin B. - Helv. chim. Acta43 2129, 1960.

    Google Scholar 

  3. Dixit P. K., Lazarow A.: EDTA (Ethylene Diamine Tetraacetic Acid) Potentiation of Insulin Activity as Assayed by the Epididymal Adipose Tissue Technique - Proc. Soc. exp. Biol. (N.Y.)118 368, 1965.

    Google Scholar 

  4. Dole V. P.: A Relation between non-Esterified Fatty Acids in Plasma and the Metabolism of Glucose - J. clin. Invest.35 150, 1956.

    PubMed  Google Scholar 

  5. Flatt J. P., Ball E. G.: Studies on the Metabolism of Adipose Tissue. XV. An Evaluation of the Major Pathways of Glucose Catabolism as Influenced by Insulin and Epinephrine -J. biol. Chem.239 675, 1964.

    PubMed  Google Scholar 

  6. Frey F.: Erfahrungen mit Desferrioxamin bei pathologischen Eisenablagerungen - Gespräch am runden Tisch - Schweiz. med. Wschr.92 1295, 1962.

    Google Scholar 

  7. Ghidoni A., Sanesi E., Pozza G.: Effect of Desferrioxamine Methansulphonate (DFOM) on Glucose Uptake by Rat Epididymal Adipose Tissue - Proc. Soc. exp. Biol. (N.Y.)121 1039, 1966.

    Google Scholar 

  8. Goldstein M. S., Henry W. R., Huddlestun B., Levine R.: Action of Insulin on Transfer of Sugar across Cell Barriers: Common Chemical Configuration of Substances to Action of the Hormone - Amer. J. Physiol.173 207, 1953.

    PubMed  Google Scholar 

  9. Goucher C. R., Taylor J. F.: Compounds of Ferric Iron with Adenosine Triphosphate and other Nucleosides Phosphates - J. biol. Chem.239 2251, 1964.

    PubMed  Google Scholar 

  10. Hugget A. S., Nixon D. A.: Enzymic Determination of Blood Glucose -Biochem. J.66 12, 1957.

    Google Scholar 

  11. Jeanrenaud B.: Fonctions et régulation hormonale du tissu adipeux - Helv. med. Acta30 1, 1963.

    Google Scholar 

  12. Krebs H. A., Gevers W.: Experiments on the Role of Adenosine Monophosphate in the Regulation of Carbohydrate Metabolism in Pigeon - Biochem. J.97 1, 1965.

    Google Scholar 

  13. Krebs H. A., Woodford M.: Fructose-1,6-Diphosphatase in Striated Muscle - Biochem. J.94 436, 1965.

    PubMed  Google Scholar 

  14. Landau B. R., Katz J.: A Quantitative Estimation of Pathways of Glucose Metabolism in Rat Adipose Tissue in Vitro - J. biol. Chem.239 697, 1964.

    PubMed  Google Scholar 

  15. Leonards J. R., Landau B. R., Bartsch G.: Assay of Insulin and Insulin-like Activity with Rat Epididymal Fat Pad - J. Lab. clin. Med.60 552, 1962.

    PubMed  Google Scholar 

  16. Petrella A.: Comunicazione personale.

  17. Pozza G., Eridani S.: A Study of the Isolated Rat Diaphragm Technique for Estimating Insulin Effect - Clin. chim. Acta4 889, 1959.

    PubMed  Google Scholar 

  18. Roe J. H.: A Colorimetric Method for the Determination of Fructose in Blood and Urine -J. biol. Chem.107 15, 1934.

    Google Scholar 

  19. Steinke J., Soeldner J. S., Camerini-Davalos R. A., Renold A. E.: Studies on Serum Insulin-Like Activity (ILA) in Prediabetes and Early Overt Diabetes - Diabetes12 502, 1963.

    PubMed  Google Scholar 

  20. Underwood A. H., Newsholme E. A.: Some Properties of Fructose-1-6-Diphosphatase of Rat Liver and their Relation to the Control of Gluconeogenesis - Biochem. J.95 767, 1965.

    PubMed  Google Scholar 

  21. Winegrad A. I., Renold A. E.: Studies on Rat Adipose Tissue in Vitro. II. Effects of Insulin on the Metabolism of Specifically Labeled Glucose - J. biol. Chem.233 273, 1958.

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Istituto di Clinica Medica Generale e Terapia Medica dell'Universita' di Milano, Via F. Sforza, 35, Milano, Italy

    Guido Pozza, Alberto Ghidoni, Giuseppe Sorgato & Edda Sanesi

Authors
  1. Guido Pozza
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Alberto Ghidoni
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Giuseppe Sorgato
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Edda Sanesi
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Pozza, G., Ghidoni, A., Sorgato, G. et al. Studio del metabolismo glicidico nel tessuto adiposo e nel muscolo in rapporto all'azione di un agente chelante, la desferrioxamina. Acta diabet. lat 3, 381–391 (1966). https://doi.org/10.1007/BF01545400

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01545400

Search

Navigation