Abstract
An immunoelectron microscopy study of human T-lymphotropic virus (HLTV) type IIa- and type IIb-producing cells was performed to clarify lymphocyte marker expression including that of T-cell receptor (TCR). Although an immune reaction with TCR antibody β-chain was detected in the rough endoplasmic reticulum, but not the plasma membrane (PM), staining for TCR α-chain antibody was mostly negative in HLTV-IIa- and IIb-producing cells. Moreover. TCR α/β antibody yielded no positive reaction in HLTV-IIa- and-IIb-producing cells, including PM. Both the PM and nuclear membrane in HLTV-IIa- and-IIb-producing cells were positive for CD3; CD25 and HLA-DR were positive for the PM of HLTV-IIa- and-IIb-producing cells. These findings suggest that HLTV-IIa- and-IIb-producing cells are activated T cells expressing CD3, CD25, and HLA-DR antigens, but are immune deficient for antigen recognition because of defective expression of TCR-CD3 complex.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Ohtsuki Y, Iwata J, Oka T, Furihata M, Sonobe H, Miyoshi I (1991) Immunoelectron microscopic study of HTLV-I-producing human lymphocytes in vitro. Proc Am Assoc Cancer Res 32:313
Ohtsuki Y, Iwata J, Furihata M, Sonobe H (1991) Immunoelectron microscopic study of HTLV-I-producing cells on the expression of lymphocyte markers. J. Clin Electron Microse 24:469–470
Ohtsuki Y, Iwata J, Furihata M, Sonobe H, Miyoshi I (1992) Electron and immunoelectron microscopic studies on human T-lymphotropic virus type I (HTLV-I)-related diseases. J Clin Electron Microsc 25:358–359
Ohtsuki Y, Miyoshi I (1994) Ultrastructural studies on human T-lymphotropic virus type I-related diseases (in Japanese). Electron Microsc 29:18–25
Suzushima H, Hattori T, Ason N, Wang JX, Nishikawa K, Okubo T, Anderson P, Takatsuki K (1991) Discordant gene and surface expression of the T-cell receptor/CD3 complex in adult T-cell leukemia cells. Cancer Res 51:6084–6088
Suzushima H, Asou N, Nishimura S, Nishikawa K, Wang JX, Okubo T, Naito M, Hattori T, Takatsuki K (1993) Double-negative (CD4-CD8-) T cells from adult T-cell leukemia patients also have poor expression of the T-cell receptor αβ/CD3 complex. Blood 81:1032–1039
Ohara N, Hayashi K, Miyamoto K, Tomita N, Fujiwara K, Kondo E, Takahashi K, Ohtsuki Y, Akagi T (1993) A human T cell line with an abnormal trisomy 2 karyotype established by coculture of peripheral lymphocytes with an HTLV-II-infected simian leukocyte cell line. Acta Pathol Jpn 43:237–243
Brenner MB, McLean J, Scheft, H, Warnke RA, Jones N, Strominger JL (1987) Characterization and expression of the human αβ T-cell receptor by using a framework monoclonal antibody. J Immunol 138:1502–1509
Furley AJ, Mizutani S, Weilbaecher K, Dhaliwal HS, Ford AM, Chan LC, Molgaard HV, Toyonaga B, Mak T, van den Elsen P, Gold D, Terhorst C, Greaves MG (1986), Developmentally regulated rearrangement and expression of genes encoding the T cell receptor-T3 complex. Cell 46:75–87
Hollema H, Poppema S (1989) T-lymphoblastic and peripheral T-cell lympomas in the northern part of the Netherlands. Cancer (Phila) 64:1620–1628
Borowitz MJ, Reichert TA, Brynes RK, Cousar JB, Whitcomb CC, Collins RD, Crissman JD, Bryne GE (1986) The phenotypic diversity of peripheral T-cell lymphomas: the Southeastern Cancer Study Group Experience. Hum Pathol 17:567–574
Matsuoka M, Hattori T, Chosa T, Tsuda H, Kuwata S, Yoshida M, Uchiyama T, Takatsuki K (1986) T3 surface molecules on adult T cell leukemia cells are modulated in vivo. Blood 67:1070–1076
Mori N, Oka K, Yoda Y, Abe T, Kojima M (1990) T-cell receptor expression in the T-cell malignancies. Am J Clin Pathol 93:495–501
Saito T, Weiss A, Gunter KC, Shevach EM, Germain N (1987) Cell surface T3 expression requires the presence of both alpha- and beta-chains of the T-cell receptor. J Immunol 139:625–628
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Ohtsuki, Y., Hayashi, K., Ohara, N. et al. Immunoelectron microscopy study on the expression of T-cell markers in human T-lymphotropic virus type II-(HTLV-II-) producing cells. Med Electron Microsc 30, 125–130 (1997). https://doi.org/10.1007/BF01545313
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01545313