Abstract
Polyethylene glycol (PEG) induces the hybridization of mammalian cells at a much higher frequency when the cells are attached to a substrate during treatment than when the cells are treated in suspension. Since many cell types, e.g., lymphocytes, cannot attach to a substrate, a new technique for the PEG-induced fusion of cells in suspension was developed. This technique, referred to as “pancake fusion,” is based on the centrifugation of suspended cells onto a coverslip and the PEG treatment of the cells on the coverslip as if they were attached to a substrate. With this technique, the frequency of hybridization of human white blood cells, which are incapable of attaching to a substrate, can be greatly increased.
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O'Malley, K.A., Davidson, R.L. A new dimension in suspension fusion techniques with polyethylene glycol. Somat Cell Mol Genet 3, 441–448 (1977). https://doi.org/10.1007/BF01542972
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DOI: https://doi.org/10.1007/BF01542972