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Induction of supermelanin synthesis and morphological changes in interspecific reconstituted cells and its reversal by tumor promoter

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Somatic Cell Genetics

Abstract

Chloramphenicol-resistant (CAP r) reconstituted cells and cybrids were isolated by fusion of karyoplasts (or intact cells) of mouse amelanotic melanoma B16 cells with cytoplasts of hypoxanthine-guanine phosphoribosyltransferase (HGPRT)-deficient, CAPr rat myoblastic cells, L6TG.CAPr, and double selection in HAT medium containing CAP. Reconstituted cells or cybrids exhibited unique cellular arrangement, and about one third of the isolated clones expressed high tyrosinase activity and marked melanin synthesis, although the parental mouse cells expressed low tyrosinase activity and the parental rat cells did not express tyrosinase activity. These phenotypic changes have been stable for more than a year. The phenotypic reversions of these clonal cells were induced by treatment with a tumor promoter. There were changes in the morphology of the treated cells to that of the mouse B16 cells and extinction of tyrosinase activity and melanin synthesis in pigmented clonal cells. These phenotypic changes and reversions induced by a promoter were repeatedly reversible.

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Authors and Affiliations

  1. Radiobiology Division, National Cancer Center Research Instiute, Tokyo, Japan

    Toyozo Sekiguchi & Mariko Tosu

  2. Chromosome Research Unit, Faculty of Science, Hokkaido University, Sapporo, Japan

    Michihiro C. Yoshida

  3. Institute for Tuberculosis and Cancer, Tohoku University, Sendai, Japan

    Atsushi Oikawa

  4. Dermatology Division, National Cancer Center Hospital, Tokyo, Japan

    Kazuyuki Ishihara

  5. High-Risk Study Division, National Cancer Center Research Institute, Tokyo, Japan

    Hirota Fujiki

  6. Pathology Division, National Cancer Center Research Institute, Tokyo, Japan

    Masaru Tumuraya & Toru Kameya

Authors
  1. Toyozo Sekiguchi
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  2. Mariko Tosu
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  3. Michihiro C. Yoshida
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  4. Atsushi Oikawa
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  5. Kazuyuki Ishihara
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  6. Hirota Fujiki
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  7. Masaru Tumuraya
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  8. Toru Kameya
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Sekiguchi, T., Tosu, M., Yoshida, M.C. et al. Induction of supermelanin synthesis and morphological changes in interspecific reconstituted cells and its reversal by tumor promoter. Somat Cell Mol Genet 8, 605–622 (1982). https://doi.org/10.1007/BF01542854

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  • DOI: https://doi.org/10.1007/BF01542854

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