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Formation of hybridoma clones in soft agarose: Effect of pH and of medium

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Somatic Cell Genetics

Abstract

Optimal conditions for the formation of hybridoma clones in soft agarose are described. The hybridization frequency is shown to be highly dependent on the pH of the polyethylene glycol (PEG) solution used for fusion and on the cloning medium. Maximal numbers of clones are obtained when the PEG solution used for fusion is at pH 8.0–8.2.

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Authors and Affiliations

  1. Department of Microbiology, Cancer Center/Institute of Cancer Research, College of Physicians and Surgeons, Columbia University, 701 West 168th St., 10032, New York, New York

    Jacqueline Sharon, Sherie L. Morrison & Elvin A. Rabat

  2. Department of Human Genetics and Development, Cancer Center/Institute of Cancer Research, College of Physicians and Surgeons, Columbia University, 701 West 168th St., 10032, New York, New York

    Elvin A. Rabat

  3. Department of Neurology, Cancer Center/Institute of Cancer Research, College of Physicians and Surgeons, Columbia University, 701 West 168th St., 10032, New York, New York

    Elvin A. Rabat

Authors
  1. Jacqueline Sharon
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  2. Sherie L. Morrison
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  3. Elvin A. Rabat
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Sharon, J., Morrison, S.L. & Rabat, E.A. Formation of hybridoma clones in soft agarose: Effect of pH and of medium. Somat Cell Mol Genet 6, 435–441 (1980). https://doi.org/10.1007/BF01542794

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  • DOI: https://doi.org/10.1007/BF01542794

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