Abstract
The acquisition of high-level resistance to tubercidin (an adenosine analog) in CHO cells occurs in a single step at high frequency (10−3 to 10−4) without mutagenesis. Analysis of a large number of independent mutants by a fluctuation test (Luria and Delbruck, 1943) indicates that they arise independently of the selection medium and all fall into the same complementation group. All mutants tested lack detectable adenosine kinase activity. An analysis of hybrids between mutant and wild-type cells indicates that resistance to tubercidin is a recessive marker which segregates as would be expected if it were a haploid locus in the parental CHO cell. Resistance to tubercidin is not linked to the X chromosome in CHO cells and appears to occur at much lower frequency in primary Chinese hamster cells and other cultured cell lines.
Similar content being viewed by others
Literature cited
Siminovitch, L. (1976).Cell 7:1–11.
Suhadolnik, R. J. (1970).Nucleoside Antibiotics (Wiley-Interscience, New York), pp. 298–353.
Gupta, R. S., and Simonovitch, L. (1978).Somat. Cell Genet.4:715–735.
Puck, T. T., Ciecuira, S. J., and Robinson, A. (1958).J. Exp. Med. 108:945–955.
Stanley, P., Callibot, V., and Siminovitch, L. (1975).Cell 6:121–128.
Thompson, L. H., and Baker, R. M. (1973). InMethods in Cell Biology, Vol. 6, (ed.) Prescott, D. (Academic Press, New York), pp. 209–281.
McBurney, M. W., and Whitmore, G. F. (1974).Cell 2:173–182.
Worton, R. G., Ho, C. C., and Duff, C. (1977).Somat. Cell Genet. 3:27–45.
Chan, T., Creagan, R. P., and Reardon, M. P. (1978).Somat. Cell Genet. 4:1–12.
Pontecorvo, G. (1975).Somat. Cell Genet. 1:397–400.
Davidson, R. L., and Gerald, P. S. (1976).Somat. Cell Genet. 2:165–176.
Davidson, R. L., O'Malley, K. A., and Wheeler, T. A. (1976).Somat. Cell Genet. 2:271–280.
Jha, K. K., and Ozer, H. L. (1976).Somat. Cell Genet. 2:215–223.
Chan, T.-S., Ishii, K., Long, C., and Green, H. (1973).J. Cell Physiol. 81:315–322.
Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J. (1951).J. Biol. Chem. 193:265–275.
Luria, S. E., and Delbruck, M. (1943).Genetics 28:491–511.
Chasin, L. (1972).Nature (London), New Biol. 240:50–52.
Bukhari, A. I., Shapiro, J. A., and Adhya, S., eds. (1977).DNA Insertion Elements, Plasmids, and Episomes (Cold Spring Harbor Laboratory, Cold Spring Harbor, New York).
Zieg, J., Silverman, M., Hilmen, M., and Simon, M. (1977).Science 196:170–172.
Rosner, J. L., and Gottesman, M. M. (1977). InDNA Insertion Elements, Plasmids, and Episomes (eds.) Bukhari, A. I., Shapiro, J. A., and Adhya, S. (Cold Spring Harbor Laboratory, Cold Spring Harbor, New York), pp. 213–218.
Gottesman, M. E., and Weisberg, R. A. (1971). InThe Bacteriophage Lambda (ed.) Hershey, A. D. (Cold Spring Harbor Laboratory, Cold Spring Harbor, New York), pp. 113–138.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Rabin, M.S., Gottesman, M.M. High frequency of mutation to tubercidin resistance in CHO cells. Somat Cell Mol Genet 5, 571–583 (1979). https://doi.org/10.1007/BF01542695
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF01542695