Abstract
An untransformed parental Chinese hamster cell line, one polyoma transformed clone, and two clones containing both SV40 and polyoma DNA were tested for the appearance of variants resistant to 6-thioguanine or oubain. The frequency of such variants was found to be highest in doubly transformed cells. The mutation rate at these loci was correlated with the level of transformation. The mutants observed were stable and had low reversion frequencies with no gross cytogenetic changes. These results establish a quantitative basis for the evaluation of phenotypic variability observed in transformed cell populations.
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Literature cited
McCann, J., and Ames, B. (1976).Proc. Natl. Acad. Sci. U.S.A. 73:950–954.
Barrett, J. C., and Ts'o, P. O. (1978).Proc. Natl. Acad. Sci. U.S.A. 75:3297–3301.
Huberman, E., Mager, R., and Sachs, L. (1976).Nature 264:360–361.
Landolph, J. R., and Heidelberger, C. (1979).Proc. Natl. Acad. Sci. U.S.A. 76:930–934.
Wolman, S. R., Hirschhorn, K., and Todaro, G. J. (1964).Cytogenetics 3:45–61.
Lehman, J. M. (1974).Int. J. Cancer 13:164–172.
Marshak, M. I., Vanshaver, N. B., and Shapiro, M. I. (1975).Mutat. Res. 30:383–396.
Theile, M., Scherneck, S., and Geissler, E. (1976).Mutat. Res. 37:111–124.
Hirai, K., Lehman, J. M., and Defendi, V. (1971).J. Virol. 8:708–715.
Boram, W., and Abelson, J. (1971).J. Mol. Biol. 62:171–178.
Wolman, S. R., and Horland, A. A. (1975).In Cancer, Vol. 3, (ed.) Becker, F. (Plenum, New York), pp. 155–198.
Macpherson, I., and Montagnier, L. (1964).Virology 23:291–294.
Nardone, R. M., Todd, G., Gonzalez, P., and Gaffney, E. V. (1965).Science 149:1100–1101.
Chasin, L. A. (1973).J. Cell. Physiol. 82:299–308.
Luria, S., and Delbrück, M. (1943).Genetics 28:491–511.
Burton, K. (1956).Biochem. J. 62:315–323.
Littlefield, J. (1964).Science 145:709–710.
Siminovitch, L. (1976).Cell 7:1–12.
Cox, E. C. (1976).Annu. Rev. Genet. 10:135–156.
Cox, E. C. (1970).J. Mol. Biol. 50:129–135.
Coukell, N. B., and Yanofsky, C. (1970).Nature 228:633–635.
Martin, R. G., and Oppenheimer, A. (1977).Cell 11:859–869.
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Goldberg, S., Defendi, V. Increased mutation rates in doubly viral transformed Chinese hamster cells. Somat Cell Mol Genet 5, 887–895 (1979). https://doi.org/10.1007/BF01542648
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DOI: https://doi.org/10.1007/BF01542648