Skip to main content
SpringerLink
Log in

Autologous human B-Cell immune response to pulmonary adenocarcinomatous polymorphic epithelial mucin

  • Original Articles
  • Published:
Journal of Clinical Immunology Aims and scope Submit manuscript

Abstract

In order to investigate whether a B-cell immune response to the polymorphic epithelial mucin (PEM) is present in adenocarcinomatous patients, peripheral blood lymphocytes from 20 patients with adenocarcinomas from various sites were fused with the mouse-human heteromyeloma cell line SHM-D33. One IgM(K) monoclonal antibody derived from peripheral blood lymphocytes of a patient with a lung adenocarcinoma was generated with binding reactivity for bovine submaxillary mucin (BSM). The latter is one of the PEMs, and it contains a large amount of the tumor-associated sialosyl-Tn epitope. The generated antibody was designated KMD-2. Immunohistological studies on various tissues showed that KMD-2 reacted with 6/6 colorectal adenocarcinomas and 4/6 pulmonary adenocarcinomas. The antibody also displayed a slight degree of cross-reactivity with a limited number of normal tissues, especially those that elaborate mucin. Our data show that an autologous antibody response in the form of IgM to the immunosuppressive PEM is present in some adenocarcinomatous patients. The data also suggest that the KMD-2 (IgM/K) antibody may be of clinical importance for diagnostic purposes of adenocarcinomatous disease.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Devine P, Mckenzie F: Mucins: Structure, function and associations with malignancy. BioAssays 14:619–625, 1992

    Google Scholar 

  2. Gendler S, Lancaster C, Duhig T, Peat N, Burchell J, Pemberton L, Lalani E, Wilson D: Molecular cloning and expression of human tumor-associated polymorphic epithelial mucin. J Biol Chem 265:15286–15293, 1990

    PubMed  Google Scholar 

  3. Stahli C, Caravatti M, Aeschbacher M, Kocyba C, Takacs B, Carmann H: Mucin-like carcinoma-associated antigen defined by three monoclonal antibodies against different epitopes. Cancer Res 46:6799–6802, 1988.

    Google Scholar 

  4. Dahiya R, Kwak K, Byrd J, Ho S, Yoon W, Kim Y: Mucin synthesis and secretion in various human epithelial cancer cell lines that express theMUC-1 mucin gene. Cancer Res 53:1437–1443, 1993

    PubMed  Google Scholar 

  5. Thor A, Ohuchi N, Szpak C, Johnston W, Schlom J: Distribution of oncofetal antigen tumor-associated glycoprotein-72 defined by monoclonal antibody B72.3. Cancer Res 46:3118–3124, 1986

    PubMed  Google Scholar 

  6. Tsuji T, Osawa T: Carbohydrate structures of bovine submaxillary mucin. Carbohydr Res 151:391–402, 1986

    PubMed  Google Scholar 

  7. Kjeldsen T, Clausen H, Hirohashi S, Ogawa T, Iijima H, Hakomori S: Preparation and characterization of monoclonal antibodies directed to the tumor-associated O-linked sialosyl-2-6α-N-acetylgalactosaminyl (sialosyl-Tn) epitope. Cancer Res 48:2214–2220, 1988

    PubMed  Google Scholar 

  8. Xiang J, Chen Z: Genetic engineering of high affinity anti-human colorectal tumor mouse/human chimeric antibody. Immunology 75:209–216, 1992

    PubMed  Google Scholar 

  9. Xiang J, Moyana T, Chen Z, Han W, Ping T, Li E: Production and characterization of a tumor-specific monoclonal antibody ACT19 recognizing an epitope distinctive from sialosyl-Tn on the TAG72 antigen. Tumori 79:58–65, 1993

    PubMed  Google Scholar 

  10. Fung P, Longenecker B: Specific immunosuppressive activity of epiglycanin, a mucin-like glycoprotein secreted by a murine mammary adenocarcinoma (TA3-HA). Cancer Res 51:1170–1176, 1991

    PubMed  Google Scholar 

  11. Ogata S, Maimonis P, Itzkowitz S: Mucins bearing the cancerassociated sialosyl-Tn antigen mediate inhibition of natural killer cell cytotoxicity. Cancer Res 52:4741–4746, 1992

    PubMed  Google Scholar 

  12. Kemenade E, Ligtenberg J, Boer A, Vos H, Melief C, Hilkens J, Figdor C: Episialin (MUC1) inhibits cytotoxic lymphocyte-target cell interaction. J Immunol 151:767–776, 1993

    PubMed  Google Scholar 

  13. Teng N, Lam K, Riera F, Kaplan H: Construction and testing of mouse/human heteromyolomas for human monoclonal antibody production. Proc Natl Acad Sci USA 80:7308–7312, 1983

    PubMed  Google Scholar 

  14. Xiang J, Moyana T, Kalra J, Hamilton T, Qi Y: Construction and characterization of a high-affinity chimeric anti-colorectal carcinoma antibody ccM4. Mol Biother 4:174–182, 1992

    PubMed  Google Scholar 

  15. Rughetti A, Turchi V, Ghetti C, Scambia G, Panici P, Roncucci G, Mancuso M, Frati L, Nuti M. Human B-cell immune response to the polymorphic epithelial mucin. 53:2457–2459, 1993

    Google Scholar 

  16. Paul W, Seder R. Lymphocyte responses and cytokines. Cell 76:241–251, 1994

    PubMed  Google Scholar 

  17. Kalofonos H, Stewart S, Epenetos A: Antibody-guided diagnosis and therapy of malignant lesions. Int J Cancer 2:74–80, 1988

    Google Scholar 

  18. Levy R, Miller R: Biological and clinical implications of lymphocyte hybridomas: tumor therapy with monoclonal antibodies. Annu Rev Med 34:107–127, 1983

    PubMed  Google Scholar 

  19. Jothy S, Brazinsky S, Loy M, Haggarty A, Krantz M, Cheung M, Fuks A: Characterization of monoclonal antibodies to carcinoem-bryonic antigen with increased tumor specificity. Lab Invest 54:108–117, 1986

    PubMed  Google Scholar 

  20. Wolf B, Salem R, Sears H, Horst D, Lavin P, Herlyn M: The expression of colorectal carcinoma-associated antigen in the normal colonic mucosa: An immunohistochemical analysis of regional distribution. Am J Pathol 135:111–119, 1989

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Saskatoon Cancer Center, Division of Oncology, University of Saskatchewan, 20 Campus Drive, S7N 4H4, Saskatoon, Saskatchewan, Canada

    J. Xiang & A. Maksymiuk

  2. Department of Pathology, University of Saskatchewan, 20 Campus Drive, S7N 4H4, Saskatoon, Saskatchewan, Canada

    T. Moyana

Authors
  1. J. Xiang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. T. Moyana
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. A. Maksymiuk
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Xiang, J., Moyana, T. & Maksymiuk, A. Autologous human B-Cell immune response to pulmonary adenocarcinomatous polymorphic epithelial mucin. J Clin Immunol 15, 74–79 (1995). https://doi.org/10.1007/BF01541735

Download citation

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01541735

Key words

Search

Navigation