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Biochemical patterns in brain tumours

III. Transfer ribonucleic acid

Biochemische Untersuchungen bei Hirntumoren

III. Transfer-Ribonuklein-Säure

Études biochimiques dans les tumeurs cerebrales

III. Acides ribonucleiques de transfert

Ricerche biochimiche sui tumori cerebrali

III. Acido ribonucleico „transfer“

Estudios bioquímicos en los tumores cerebrales

III. Acido ribonucleico “transfer”

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Summary

Unfractionated t-RNAs from brain tumours contain higher amounts of methylated bases and 2′-O-riboses, compared to normal human brain; the methylated base ratios are significantly altered. The secondary structure of the tumoural t-RNAs differs, mainly in relation to the base pairing. An hypothetical schema of the role of the modifications of the primary and secondary structures of the t-RNA in the metabolism of the tumour cell is proposed. It is assumed that a loss of specificity of the methylase activities occurs in brain tumours.

Zusammenfassung

Unfraktionierte t-RNS von Hirntumoren enthalten mehr methylierte Basen und 2′-O-Ribosen als das normale menschliche Hirngewebe. Das Verhältnis der methylierten Basen zueinander ist bedeutsam verändert. Die Sekundärstruktur der tumoralen t-RNS variiert hauptsächlich in Abhängigkeit von der Basenpaarung. In einem hypothetischen Schema wird dargelegt, welche Rolle die Veränderungen der Primär- und Sekundärstrukturen der t-RNS im Stoffwechsel der Tumorzellen spielen. Man nimmt an, daß in Hirntumoren ein Spezifizitätsverlust der Methylaseaktivität vorliegt.

Résumé

Les acides ribonucléiques de transfert non fractionnés, provenant de tumeurs cérébrales, sont plus riches en bases et en riboses 2′-O-méthylés, par comparaison avec ceux du tissu cérébral humain normal;les rapports des bases méthylées entre elles sont modifiés de façon significative. La structure secondaire des ARN de transfert des tumeurs diffère, surtout au niveau des interactions base-base. Un schéma hypothétique du rôle que les modifications des structures primaires et secondaires de l'ARN de transfert joue dans le métabolisme des tumeurs est exposé. On admet qu'il existe une perte de spécificité des activités méthylasiques dans certaines tumeurs cérébrales.

Riassunto

t-ARN non frazionati di tumori cerebrali contengono quantità maggiori di basi e 2′-O-ribosi metilati, ripsetto al cervello umano normale; i rapporti fra le basi metilate sono significativamente alterati. La struttura secondaria dei t-ARN tumorali differisce, in rapporto soprattutto con gli aecoppiamenti delle basi. Uno schema ipotetico del ruolo svolto dalle modificazioni a carico delle strutture primaria e secondaria dei t-ARN nel metabolismo tumorale é esposto. Si ammette ehe una perdita di specificità delle reazioni metilasiche ricorre in certi tumori cerebrali.

Resumen

Los t-ARN no fraccionados de los tumores cerebrales son más ricos en bases y 2′-O-riboses metiladas, en comparación con el cerebro humano normal; las relaciones entre las bases metiladas estan alteradas muy significativamente. La estructura seoundaria de las t-ARN de los tumores difiere en relación, sobre todo, con las interacciones base-base. Se expone un esquema hipotético del papel que las modificaciones de las estructuras primaria y secundaria del t-ARN juegan en el metabolismo de los tumores. Se admite una pérdida de especificidad de las actividades metilásicas en ciertos tumores cerebrales.

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  1. G. L. Viale

    Present address: Laboratory for Brain Tumour Research, Neurosurgical Clinic of the University of Genoa, Italy

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    Viale, G.L. Biochemical patterns in brain tumours. Acta neurochir 21, 123–137 (1969). https://doi.org/10.1007/BF01540920

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