Skip to main content

Maintenance of the EHS sarcoma and Matrigel preparation


Matrigel is a reconstituted basement membrane extract which is rich in laminin, growth factors, entactin/nidogen, type IV collagen, and heparan sulfate proteoglycan (perlecan). This basement membrane extract can be used for promotion and maintenance of a differentiated phenotype in cell cultures, as well as for a variety of in vitro and in vivo uses. The Engelbreth-Holm-Swarm (EHS) sarcoma is a rich source of both individual basement membrane components and Matrigel. Maintenance of the EHS tumor in mice and its subsequent harvesting for the preparation of Matrigel are described.

This is a preview of subscription content, access via your institution.


  1. Albini A, Iwamoto Y, Kleinman HK, Martin GR, Aaronson SA, Kozlowski JM, McEwan RN (1987). A rapid in vitro assay for quantitating the invasive potential of tumor cells. Cancer Res 47: 3239–3245.

    PubMed  Google Scholar 

  2. Fridman R, Kibbey MC, Royce LS, Zain M, Sweeney TM, Jicha DL, Yannelli JR, Martin GR, Kleinman HK (1991). Enhanced tumor growth of both primary and established human and murine tumor cells in athymic mice after coinjection with Matrigel. J Natl Cancer Inst 83: 769–774.

    PubMed  Google Scholar 

  3. Hay ED, ed. (1991). Cell biology of extracellular matrix. New York: Plenum Press.

    Google Scholar 

  4. Kibbey MC, Grant DS, Kleinman HK (1992). Role of the SIKVAV site of laminin in promotion of angiogenesis and tumor growth: An in vivo Matrigel model. J Natl Cancer Inst 84: 1633–1638.

    PubMed  Google Scholar 

  5. Kleinman HK, Kibbey MC, Schnaper HW, Hadley MA, Dym M, Grant DS (1993). Role of basement membrane in differentiation. In: Rohrbach DA, Timpl R, eds, Molecular and cellular aspects of basement membranes. New York: Academic Press, pp 309–326.

    Google Scholar 

  6. Kleinman HK, McGarvey ML, Hassell JR, Star VL, Cannon FB, Laurie GW, Martin GR (1986). Basement membrane complexes with biological activity. Biochemistry 25: 312–318.

    PubMed  Google Scholar 

  7. Orkin RW, Gehron P, McGoodwin EB, Martin GR, Valentine T, Swarm R (1977). A murine tumor producing a matrix of basement membrane. J Exper Med 145: 204–224.

    Google Scholar 

Download references

Author information

Authors and Affiliations


Rights and permissions

Reprints and Permissions

About this article

Cite this article

Kibbey, M.C. Maintenance of the EHS sarcoma and Matrigel preparation. Journal of Tissue Culture Methods 16, 227–230 (1994).

Download citation

  • Issue Date:

  • DOI:

Key words

  • Basement membrane
  • EHS sarcoma
  • Matrigel