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Distribution of a novel pituitary protein (7B2) in mammalian gastrointestinal tract and pancreas

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Abstract

The distribution of a novel pituitary protein (7B2) was determined in the gastrointestinal tract and pancreas of four mammalian species (man, pig, guinea pig, and rat) by a specific radioimmunoassay. The highest concentrations of cross-reacting immunoreactive 7B2 (IR-7B2) were observed in the pancreas and the proximal gut (antrum or duodenum). While the intestinal concentrations varied widely among species, pancreatic IR-7B2 concentrations appeared to be similar in all four species. In the rat, pancreatic islets were found to contain high concentrations of IR-7B2 (5.73± 0.14 fmol/islet, mean± sem). Neonatal capsaicin treatment and enteric nerve section did not affect the concentrations of IR-7B2 in the rat intestine. Layer separation of human gut showed that IR-7B2 is mainly (71± 8%) present in the epithelial fraction. Chromatographic analysis of intestinal and pancreatic extracts from the four species on Sephadex G-100 showed the presence of two immunoreactive peaks at Kav 0.3 and 0.6, but there were both inter- and intraspecies variations in the proportions of the larger and smaller molecular forms.

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Authors and Affiliations

  1. Departments of Medicine and Histochemistry, Royal Postgraduate Medical School, Hammersmith Hospital, DuCane Road, W12 0HS, London, UK

    H. Suzuki, N. D. Christofides, M. Ghiglione, G. -L. Ferri, M. Chretien, N. G. Seidah, J. M. Polak &  S. R. Bloom

  2. Department of Anatomy, Tor Vergata University, via Orazio Raimondo, 00173, Rome, Italy

    H. Suzuki, N. D. Christofides, M. Ghiglione, G. -L. Ferri, M. Chretien, N. G. Seidah, J. M. Polak &  S. R. Bloom

  3. Laboratories of Molecular and Biochemical Neuroendocrinology, Clinical Research Institute of Montreal, 110 Pine Avenue West, H2W 1R7, Montreal, Canada

    H. Suzuki, N. D. Christofides, M. Ghiglione, G. -L. Ferri, M. Chretien, N. G. Seidah, J. M. Polak &  S. R. Bloom

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  1. H. Suzuki
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  2. N. D. Christofides
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  3. M. Ghiglione
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  4. G. -L. Ferri
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  5. M. Chretien
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  6. N. G. Seidah
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  7. J. M. Polak
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  8. S. R. Bloom
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Suzuki, H., Christofides, N.D., Ghiglione, M. et al. Distribution of a novel pituitary protein (7B2) in mammalian gastrointestinal tract and pancreas. Digest Dis Sci 33, 718–723 (1988). https://doi.org/10.1007/BF01540436

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  • DOI: https://doi.org/10.1007/BF01540436

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