Abstract
Peptide expression influenced by human chromosome 21 was examined by comparing two-dimensional electrophoretograms of a human-hamster hybrid cell containing human chromosome 21 with its parent hamster cell and a revenant of the hybrid which had segregated the chromosome 21 genes for SOD-1, GARS, and a cytotoxic cell-surface antigen. Certain peptides were found in the hybrid but not in the hamster cell. Some, but not all, of these peptides segregated with markers for chromosome 21. Hamster peptides were also found which apparently were suppressed in the hybrid. Finally, one peptide was identified which was unique to the revertant cell. These findings may be of potential relevance to Down's syndrome.
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Scoggin, C.H., and Patterson, D. (1982).Arch. Intern. Med. 142:462–464.
Schitiu, S., Sinet, P.M., Lejeune, J., and Frezal, J. (1974).Humangenetik 23:65–72.
Feaster, W.W., Kwok, L.L., and Epstein, C.J. (1977).Am. J. Hum. Genet. 29:563–570.
Bartley, J.A., and Epstein, C.J. (1980).Biochem. Biophys. Res. Commun. 93:1286–1289.
Scoggin, C.H., Bleskan, J., Davidson, J.N., and Patterson, D. (1980).Clin. Res. 28:31A.
Weil, J., and Epstein, C.J. (1979).Am. J. Hum. Genet. 7:478–488.
Van Keuren, M., Merril, C.R., and Goldman, D. (1983). InGene Expression in Normal and Transformed Cells. Celis, J., (ed.) (Plenum Publishing New York), In press.
Moore, E.E., Jones, C., Kao, F.T., and Oates, D.C. (1977).Am. J. Hum. Genet. 29:389–396.
Miller, Y.E., Scoggin, C.H., Jones, C., and Patterson, D. (1983).J. Clin. Invest. Submitted.
Kao, F.T., Jones, C., and Puck, T.T. (1976).Proc. Natl. Acad. Sci. U.S.A. 73:193–197.
Krumlauf, R., Jeanpierre, M., and Young, B.D. (1982).Proc. Natl. Acad. Sci. U.S.A. 79:2971–2973.
Schandle, V., and Patterson, D. (1983).Bradbury Reports. In press.
Law, M.L., Davidson, J.N., and Kao, F.T. (1982).Proc. Natl. Acad. Sci. U.S.A. 79:7390–7394.
O'Farrell, P.H. (1975).J. Biol. Chem. 250:4007–4021.
McConkey, E. (1980).Somat. Cell. Genet. 6:139–147.
Kao, F.T., and Puck, T.T. (1972).Proc. Natl. Acad. Sci. U.S.A. 69:3273–3277.
Evans, H.G., Buckland, R.A., and Pardue, M.L. (1974).Chromosoma 48:405–426.
Sinet, P.M., Allard, D., Lejeune, J., and Jerome, H. (1974).C.R. Acad. Sci. D 278:3267–3270.
Patterson, D., Graw, S.L., and Jones, C. (1981).Proc. Natl. Acad. Sci. U.S.A. 78:405–409.
Epstein, C.B., and Epstein, C.J. (1976).J. Infect. Dis. 133(Suppl. A):56–62.
Vora, S., and Franke, U. (1981).Proc. Natl. Acad. Sci. U.S.A. 78:3738–3742.
Lester, S.C., Korn, H.J., and DeMars, R. (1982).Somat. Cell Genet. 8:265–284.
Paul, S.D., Fisher, J.H., and Scoggin, C.H. (1983).Clin. Res. 31:87A.
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Scoggin, C.H., Paul, S., Miller, Y.E. et al. Two-dimensional electrophoresis of peptides from human-CHO cell hybrids containing human chromosome 21. Somat Cell Mol Genet 9, 687–697 (1983). https://doi.org/10.1007/BF01539474
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DOI: https://doi.org/10.1007/BF01539474