Abstract
Hamster-human hybrids which contained an inactive human X chromosome were treated by 5-azacytidine. Hypoxanthine guanine phosphoribosyltransferase derepressed hybrids were selected and derepression of three other loci, phosphoglycerate kinase, alpha-galactosidase, and glucose-6-phosphate dehydrogenase were studied. Among 32 hybrids selected for hypoxanthine guanine phosphoribosyltransferase, two were found to be reactivated at four X loci. The independence or nonindependence of the reactivation events will be discussed. No correlation was found between the time of replication and the expression or nonexpression of the X chromosome genes: X chromosomes reactivated at four loci remained late replicating; conversely early replication can exist without the expression of some X genes.
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Hors-Cayla, M.C., Heuertz, S. & Frezal, J. Coreactivation of four inactive X genes in a hamster × human hybrid and persistence of late replication of reactivated X chromosome. Somat Cell Mol Genet 9, 645–657 (1983). https://doi.org/10.1007/BF01539470
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DOI: https://doi.org/10.1007/BF01539470