The structural gene for purine-nucleoside phosphorylase (NP) has been assigned to a subregion of chromosome 14 by somatic cell hybridization of male and female cells containing the balanced translocation t(X;14) (p22;q21). Peripheral lymphocytes were fused to a pseudodiploid HPRT-deficient established Chinese hamster cell line. 23 primary hybrid clones (10 derived from male and 13 from female cells) were isolated and maintained in HAT selective medium. Parallel subcultures from generations 16, 24, and 40 after clonal isolation were fully karyotyped and analyzed electrophoretically for expression of the human types of NP, HPRT, G6PD, and PGK. The human NP phenotype segregated discordantly with each human chromosome except chromosome 14 and the der(14),t(X;14) translocation chromosome. In all, 8 hybrids which had retained the der(X), t(X;14) translocation chromosome under HAT selective pressure and expressed human HPRT had lost the human NP phenotype. These results indicate localization of the NP gene in region 14pter→14q21.
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Francke, U., Busby, N., Shaw, D. et al. Intrachromosomal gene mapping in man: Assignment of nucleoside phosphorylase to region 14cen→14q21 by interspecific hybridization of cells with a t(X;14) (p22;q21) translocation. Somat Cell Mol Genet 2, 27–40 (1976). https://doi.org/10.1007/BF01539240
- Interspecific Hybridization
- Cell Hybridization
- Translocation Chromosome