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Biochemical and genetic evidence for a new class of emetine-resistant Chinese hamster cells with alterations in the protein biosynthetic machinery

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Somatic Cell Genetics

Abstract

We have isolated a number of emetine-resistant mutants from several different clones of CHO and CHL cells. Protein synthesis in extracts derived from each of the mutants is much more resistant to emetine than in the parental, emetine-sensitive cell lines, indicating the lesions affect the protein synthetic machinery directly. However, hybrid cell lines, derived from fusing either of two different emetine-resistant CHO mutants with either one of two different emetine-resistant CHL cells, are much more sensitive to growth inhibition by emetine than either parent. In addition, the incorporation of [3H]amino acids into protein in vivo and protein synthesis in vitro in extracts derived from these hybrids is much more sensitive to emetine inhibition than in either emetine-resistant parent. In contrast, no complementation was observed in hybrids derived from fusing two emetine-resistant CHO mutants or in hybrids derived from fusing two emetine-resistant CHL cell lines. These results indicate the CHO emetine-resistant mutants belong to one complementation group and the CHL emetineresistant mutants belong to another. The genetic loci represented by these two complementation groups must both encode for gene products involved in protein synthesis.

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Literature cited

  1. Gupta, R.S., and Siminovitch, L. (1976).Cell 9:213–219.

    PubMed  Google Scholar 

  2. Gupta, R.S., and Siminovitch, L. (1978).Somat. Cell Genet. 4:355–374.

    PubMed  Google Scholar 

  3. Moehring, J.M., and Moehring, J.J. (1979).Somat. Cell Genet. 5:453–468.

    PubMed  Google Scholar 

  4. Thompson, L.H, Lofgren, D.J., and Adair, G.M. (1977).Cell 11: 889–900.

    Google Scholar 

  5. Wasmuth, J.J., and Caskey, C.T. (1976).Cell 9:655–662.

    PubMed  Google Scholar 

  6. Boersma, D., McGill, S., Mollenkamp, J., and Roufa, D.J. (1979).J. Biol. Chem. 254:559–567.

    PubMed  Google Scholar 

  7. Gupta, R.S., and Siminovitch, L. (1977).Cell 10:61–66.

    PubMed  Google Scholar 

  8. Gupta, R.S., and Siminovitch, L. (1977).Biochemistry 16:3209–3214.

    PubMed  Google Scholar 

  9. Gupta, R.S., and Siminovitch, L. (1978).Somat. Cell Genet. 4:77–93.

    PubMed  Google Scholar 

  10. Gupta, R.S., Chan, D.Y.H., and Siminovitch, L. (1978).Cell 14:1007–1013.

    PubMed  Google Scholar 

  11. Reichenbecher, V.E., and Caskey, C.T. (1979).J. Biol. Chem. 254:6207–6210.

    PubMed  Google Scholar 

  12. Vasquez, D. (1979).Mol. Biol. Biochem. Biophys. 30:162–163.

    Google Scholar 

  13. Ford, D.K., and Yerganian, G. (1958).J. Natl. Cancer Inst. 21:393–425.

    PubMed  Google Scholar 

  14. Thompson, L.H., Harkins, J.L., and Stanners, C.P. (1973).Proc. Natl. Acad. Sci U.S.A. 70:3094–3098.

    PubMed  Google Scholar 

  15. Gillin, F.D., Roufa, D.J., Beaudet, A.L., and Caskey, C.T. (1972).Genetics 72:239–252.

    PubMed  Google Scholar 

  16. Wasmuth, J.J., and Caskey, C.T. (1976).Cell 8:71–77.

    PubMed  Google Scholar 

  17. Pelham, H.R.B., and Jackson, R.J. (1976).Eur. J. Biochem. 67:247–256.

    PubMed  Google Scholar 

  18. Villa-Komaroff, L., McDowell, M., Baltimore, D., and Lodish, H.F. (1974). InMethods Enzymol. 30:709–723.

    PubMed  Google Scholar 

  19. Boersma, D., McGill, S.M., Mollenkamp, J.W., and Roufa, D.J. (1979).Proc. Natl. Acad. Sci. U.S.A. 76:415–419.

    PubMed  Google Scholar 

  20. Campbell, C.E., and Worton, R.G. (1979).Somat. Cell Genet. 5:51–65.

    PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Biological Chemistry, California College of Medicine, University of California, Irvine, 92717, Irvine, California

    John J. Wasmuth, Janet M. Hill & Linda S. Vock

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  1. John J. Wasmuth
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  2. Janet M. Hill
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  3. Linda S. Vock
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Wasmuth, J.J., Hill, J.M. & Vock, L.S. Biochemical and genetic evidence for a new class of emetine-resistant Chinese hamster cells with alterations in the protein biosynthetic machinery. Somat Cell Mol Genet 6, 495–516 (1980). https://doi.org/10.1007/BF01539152

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  • DOI: https://doi.org/10.1007/BF01539152

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