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Increased polyethylene glycol-mediated fusion competence in mitotic cells of a mouse lymphoid cell line

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Somatic Cell Genetics

Abstract

Spontaneous mitotic cells of the mouse leukemic cell line GF7 are preferentially included in cell fusion products after treatment with polyethylene glycol (PEG). This implies a unique configuration of the natural mitotic membrane which is particularly vulnerable to induction of fusion by PEG. Colcemidarrested GF7 mitotic cells, however, are excluded from PEG-induced cell fusion products, suggesting that colcemid reverses the membrane configuration which is susceptible to the action of PEG. When Sendai virus is used as the fusogenic agent, bothcolcemidarrested and spontaneous mitotic cells are selectively fused. There must, therefore, be an essential membranefusogen reaction which is characteristically different for each of these agents.

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Authors and Affiliations

  1. Genetics Department, Iowa State University, 50011, Ames, Iowa

    D. Hansen & J. Stadler

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  1. D. Hansen
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  2. J. Stadler
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Hansen, D., Stadler, J. Increased polyethylene glycol-mediated fusion competence in mitotic cells of a mouse lymphoid cell line. Somat Cell Mol Genet 3, 471–482 (1977). https://doi.org/10.1007/BF01539119

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  • DOI: https://doi.org/10.1007/BF01539119

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