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Carbomycin resistance in mouse L cells

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Somatic Cell Genetics

Abstract

A mutant has been isolated from the mouse cell line LM(TK−) which is stably resistant to the macrolide antibiotic, carbomycin. Mitochondrial protein synthesis in this mutant was carbomycin resistant and chloramphenicol sensitive. Fusions between carbomycin-resistant and -sensitive cells produced hybrids, most of which were sensitive to 10 μg/ml carbomycin. At 7.5 μg carbomycin/ml, the average population resistance is low initially but increases with time. Carbomycin-resistant cells were enucleated and fused with carbomycin-sensitive cells under a variety of selective regimes designed to allow growth of carbomycin-resistant cytoplasmic hybrids (cybrids). No transfer of carbomycin resistance via the cytoplasm was detected. Karyoplasts from carbomycin-resistant cells showed a low transfer of resistance to 7.5 μg carbomycin/ml in karyoplast-cell fusions. Carbomycin resistance in this mutant is therefore most likely encoded in a nuclear gene.

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Authors and Affiliations

  1. Department of Human Genetics, Yale University School of Medicine, 333 Cedar Street, 06510, New Haven, Connecticut

    Clive L. Bunn & Jerome M. Eisenstadt

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  1. Clive L. Bunn
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  2. Jerome M. Eisenstadt
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Bunn, C.L., Eisenstadt, J.M. Carbomycin resistance in mouse L cells. Somat Cell Mol Genet 3, 611–627 (1977). https://doi.org/10.1007/BF01539069

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  • DOI: https://doi.org/10.1007/BF01539069

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