Skip to main content
SpringerLink
Log in

Chemically facilitated microinjection of proteins into intact monolayers of tissue culture cells

  • Published:
Somatic Cell Genetics

Abstract

Microinjection of physiologic quantities of macromolecules into tissue culture cells can facilitate the study of the biological effects of such macromolecules. In this communication, we describe a chemical technique which can be used to microinject proteins into monolayers of intact cells. Protein is loaded into erthrocyte ghosts, and the ghosts are then fused to the monolayer with polyethylene glycol 1000. Receipient cells can be injected with an efficiency of greater than 90% and contain an average of 3.8×106 microinjected molecules per cell. This technique circumvents certain problems encountered in virus-induced microinjection.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Literature cited

  1. Furusawa, M., Nishimura, T., Yamaizumi, M., and Okada, Y. (1974).Nature 249:449–450.

    PubMed  Google Scholar 

  2. Schlegel, R. A., and Rechsteiner, M. C. (1975).Cell 5:371–379.

    PubMed  Google Scholar 

  3. Wasserman, M., Zakai, N., Loyter, A., and Kulka, R. G. (1976).Cell 7:551–556.

    PubMed  Google Scholar 

  4. Kao, K. N., and Michayluk, M. R. (1974).Planta (Berlin) 115:355–367.

    Google Scholar 

  5. Pontecorvo, G. (1975).Somat. Cell Genet. 1:397–400.

    PubMed  Google Scholar 

  6. Davidson, R. L., O'Malley, K. A., and Wheeler, T. B. (1976).Somat. Cell Genet. 2:271–280.

    PubMed  Google Scholar 

  7. Hunter, W., and Greenwood, F. (1962).Nature 194:495–496.

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Division of Medical Oncology, Sidney Farber Cancer Institute, USA

    M. P. Kriegler & D. M. Livingston

  2. Departments of Medicine, Peter Bent Brigham Hospital and Harvard Medical School, Cambridge, Massachusetts

    M. P. Kriegler & D. M. Livingston

Authors
  1. M. P. Kriegler
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. D. M. Livingston
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kriegler, M.P., Livingston, D.M. Chemically facilitated microinjection of proteins into intact monolayers of tissue culture cells. Somat Cell Mol Genet 3, 603–610 (1977). https://doi.org/10.1007/BF01539068

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01539068

Keywords

Search

Navigation