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33258 Hoechst enhancement of the photosensitivity of bromodeoxyuridine-substituted cells

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Somatic Cell Genetics

Abstract

Cells that have incorporated 5-bromodeoxyuridine (BrdU) into their DNA become extremely sensitive to photoinduced killing after treatment with the dye, 33258 Hoechst. Under appropriate conditions the dye increases the efficiency of killing by a factor of approximately 104.This enhanced photosensitivity suggests that the combination of BrdU, dye, and light can be used to select conditional lethal mutants.

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Authors and Affiliations

  1. Division of Genetics, Children's Hospital Medical Center, 02115, Boston, Massachusetts

    Gail Stetten, Samuel A. Latt & Richard L. Davidson

  2. Department of Pediatrics, Harvard Medical School, 02115, Boston, Massachusetts

    Gail Stetten & Samuel A. Latt

  3. Department of Microbiology and Molecular Genetics, Harvard Medical School, 02115, Boston, Massachusetts

    Richard L. Davidson

Authors
  1. Gail Stetten
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  2. Samuel A. Latt
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  3. Richard L. Davidson
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Stetten, G., Latt, S.A. & Davidson, R.L. 33258 Hoechst enhancement of the photosensitivity of bromodeoxyuridine-substituted cells. Somat Cell Mol Genet 2, 285–290 (1976). https://doi.org/10.1007/BF01538967

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  • DOI: https://doi.org/10.1007/BF01538967

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