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Biochemical genetics of Chinese hamster cell mutants with deviant purine metabolism. IV. Isolation of a mutant which accumulates adenylosuccinic acid and succinylaminoimidazole carboxamide ribotide

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Somatic Cell Genetics

Abstract

The production, isolation, and characterization of a new complementation group (Ade−I)of adenine-requiring mutant of Chinese hamster cells (CHO-K1) is described. This mutant accumulates two intermediates of purine biosynthesis, both of which contain an aspartate moiety. One of these is shown to be adenylosuccinic acid (AMPS) by Chromatographic analysis, while evidence is presented that strongly suggests the other intermediate is succinylaminoimidazole carboxamide ribotide (SAICAR). Thus, Ade−Iis most likely lacking the activity of the enzyme adenylosuccinase (EC 4.3.2.2). The use of this and similar mutants for the analysis of regulation of purine biosynthesis in mammalian cells is discussed.

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Authors and Affiliations

  1. Eleanor Roosevelt Institute for Cancer Research, Department of Biophysics and Genetics, University of Colorado Medical Center, 80220, Denver, Colorado

    David Patterson

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  1. David Patterson
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Recipient of a Research Career Development Award, National Institute of Arthritis, Metabolism, and Digestive Diseases.

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Patterson, D. Biochemical genetics of Chinese hamster cell mutants with deviant purine metabolism. IV. Isolation of a mutant which accumulates adenylosuccinic acid and succinylaminoimidazole carboxamide ribotide. Somat Cell Mol Genet 2, 189–203 (1976). https://doi.org/10.1007/BF01538958

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  • DOI: https://doi.org/10.1007/BF01538958

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