Abstract
We have isolated 56 UV-sensitive mutant clones from a mouse L51 T/t line of L5178Y cells by a cell suspension spotting method. Five mutants have also been isolated from L51 T/t and L5178Y cells by the method reported by Thompson and coworkers (22). We divided the mutants into two groups, “highly sensitive” and “moderately sensitive” mutants, according to their sensitivity to UV irradiation. Fifty-eight mutants were highly sensitive and three were moderately sensitive to UV. The reconstruction experiments indicate that more than 90% of highly sensitive mutants were recovered by the cell suspension spotting method. Frequencies of recovered mutants highly sensitive to UV increased with increasing dose of mutagens. Recovered mutant frequency reached 10−2 after treatment with 1.5 μg/ml ofN-methyl-N′-nitro-N-nitrosoguanidine (MNNG) (survival 0.2%). Eight UV-sensitive mutants were divided into four complementation groups. These mutants were 2–6 times more sensitive to UV than parental L51 T/t cells in terms of D37 (dose required to reduce survival to 37%). Four representative UV-sensitive mutants which are classified into different complementation groups were examined for their sensitivity to killing by UV, 4-nitroquinoline-1-oxide (4NQO), mitomycin C (MMC), X-rays, and MNNG. All four classes of mutants were found to be cross-sensitive to UV, 4NQO, and MMC, but not sensitive to X-rays and MNNG.
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Literature cited
Witkin, E.M. (1976).Bacteriol. Rev. 40:869–907.
Setlow, R.B., and Carrier, W.L. (1964).Proc. Natl. Acad. Sci. U.S.A. 51:226–231.
Bridges, B.A., and Munson, R.J. (1966).Biochem. Biophys. Res. Commun. 22:268–273.
Prakash, L. (1974).Genetics 78:1101–1118.
Lawrence, C.W., and Christensen, R. (1976).Genetics 82:207–232.
Prakash, L., and Prakash, S. (1977).Genetics 86:33–55.
Nasim, A., and Smith, B.P. (1975).Genetics 79:573–582.
Pannekock, H., Noordemeer, I.A., van Sluis, C.A., and van de Putte, P. (1978).J. Bacteriol. 133:884–896.
Sancar, A., and Rupp, W.D. (1979).Biochem. Biophys. Res. Commun. 90:123–129.
Auerbach, J., and Howard-Flanders, P. (1979).Mol. Gen. Genet. 168:341–344.
Sancar, A., Stachelek, C., Konigsberg, W., and Rupp, W.D. (1980).Proc. Natl. Acad. Sci. U.S.A. 77:2611–2615.
Horii, T., Ogawa, T., and Ogawa, H. (1980).Proc. Natl. Acad. Sci. U.S.A. 77:313–317.
Horii, T., Ogawa, T., and Ogawa, H. (1981).Cell 23:689–692.
Yoakum, G.H., and Grossman, L. (1981).Nature 292:171–173.
Friedberg, E.C., Ehmann, U.K., and Williams, J.I. (1979).Adv. Radiat. Biol. 8:86–174.
Isomura, K., Nikaido, O., Horikawa, M., and Sugahara, T. (1973).Radiat. Res. 53:143–152.
Rosenstein, B., and Ohlsson-Wilhelm, B.M. (1979).Somat. Cell Genet. 5:117–128.
Schultz, R.A., Trosko, J.E., and Chang, C.C. (1981).Environ. Mutagenesis 3:53–64.
Kuroki, T., and Miyashita, S.Y. (1976).J. Cell Physiol. 90:79–90.
Stamato, T.D., and Waldren, C.A. (1977).Somat. Cell Genet. 3:431–440.
Shiomi, T., and Sato, K. (1979).Somat. Cell Genet. 5:193–202.
Thompson, L.H., Rubin, J.S., Cleaver, J.E., Whitmore, G.F., and Brookman, K. (1980).Somat. Cell Genet. 6:391–405.
Bush, D.B., Cleaver, J.E., and Glaser, D.A. (1980).Somat. Cell Genet. 6:407–418.
Sato, K., and Hieda, N. (1979).Int. J. Radiat. Biol. 35:83–87.
Sato, K., and Hieda, N. (1979).Radiat. Res. 78:167–171.
Shiomi, T., Hieda-Shiomi, N., and Sato, K. (1982).Mutat. Res. 103:61–69.
Shiomi, T., Hieda-Shiomi, N., and Sato, K. (1982).Mutat. Res. (in press).
Shiomi, T., Hieda-Shiomi, N., Sato, K., Tsuji, H., Takahashi, E., and Tobari, I. (1981).Mutat. Res. 83:107–116.
Puck, T.T., Cieciura, S.J., and Robinson, A. (1958).108:945–955.
Kuroki, T. (1973).Exp. Cell Res. 80:55–62.
Davidson, R.L., O'Malley, K.A., and Wheeler, T.B. (1976).Somat. Cell Genet. 2:271–280.
Norwood, T.H., Zeigler, C.J., and Martin, G.M. (1976).Somat. Cell Genet. 2:263–270.
Epstein, C.J. (1969).Science 163:1078–1079.
Epstein, C.J. (1972).Science 175:1467–1468.
Hashmi, S., and Miller, O.J. (1976).Cytogenet. Cell Genet. 17:35–41.
Shows, T.B., Brown, J.A., and Chapman, V.M. (1976).Cytogenet. Cell Genet. 16:436–439.
Westerveld, A., Visser, R.P., Freeke, M.A. (1972).Biochem. Genet. 7:33–40.
Shiomi, T., Hieda-Shiomi, N., and Sato, K. (1982).Mutat. Res. (in press).
Hoar, D.I., and Waghorne, C. (1978).Am. J. Hum. Genet. 30:590–601.
Wade, M.H., and Chu, E.H.Y. (1979).Mutat. Res. 59:49–60.
Sato, K., and Setlow, R.B. (1981).Mutat. Res. 84:443–455.
Schmickel, R.D., Chu, E.H.Y., Trosko, J.E., and Chang, C.C. (1977).Pediatrics 60:135–139.
Hama-Inaba, H., Hieda-Shiomi, N., Shiomi, T., and Sato, K. (1982).Mutat. Res. (in press).
Rude, J.M., and Friedberg, E.C. (1977).Mutat. Res. 42:433–442.
Inoue, M., and Takebe, H. (1978).Jpn. J. Genet. 58:285–295.
Park, S.D., and Cleaver, J.E. (1979).Nucl. Acids Res. 6:1151–1159.
Ikenaga, M., Inoue, M., Kozuka, T., and Sugita, T. (1981).Mutat. Res. 91:87–91.
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Shiomi, T., Hieda-Shiomi, N. & Sato, K. Isolation of UV-sensitive mutants of mouse L5178Y cells by a cell suspension spotting method. Somat Cell Mol Genet 8, 329–345 (1982). https://doi.org/10.1007/BF01538891
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DOI: https://doi.org/10.1007/BF01538891