Abstract
Hybrid cells produced by the fusion of pairs of cells, one a tumorigenic derivative of CHEF/16 and the other a nontumorigenic derivative of CHEF/18, give rise to clones which are largely tetraploid, but rare reduced hybrids with chromosome counts in the diploid range have been recovered from tumors of hybrid origin. This paper describes the recovery in cell culture of reduced hybrids in the diploid range by selection with 5-bromodeoxyuridine (BrdU) or methylcellulose as well as by growth in culture of cells from excised tumors. All selected subclones were tumorigenic and resistant to BrdU, but they segregated for resistance to 6-thioguanine.Unselected subclones were tetraploid, nontumorigenic, and sensitive to both drugs. These data show that chromosome reassortment as well as extensive chromosome reduction both occur in a small fraction of the population during growth of each hybrid clone.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Literature cited
Puck, T. T. (1972).The Mammalian Cell as a Microorganism-Genetic and Biochemical Studies in Vitro. Holden-Day, San Francisco, California.
Siminovitch, L. (1976).Cell 7:1–11.
Chu, E. H. Y. and Powell, S. S. (1976). InAdvances in Human Genetics, Vol. 7, (eds.) Harris, H., and Hirschhorn, K., (Plenum Press, New York), pp. 189–258.
Baker, R. M. and Ling, V. (1977). InMethods in Membrane Biology, Vol. 9, (ed.) Korn, E. D. (Plenum Press, New York), pp. 337–384.
Littlefield, J. W. (1976).Variation, Senescence, and Neoplasia in Cultured Somatic Cells. Harvard University Press, Massachusetts.
Martin, G. M., and Sprague, C. A. (1969).Science 166:761–763.
Todaro, C. J., and Green, H. (1963).J. Cell. Biol. 17:299–313;
Sager, R., and Kovac, P. E. (1978).Somat. Cell Genet. 4:375–392.
Cherington, P. V., Smith, B. L., and Pardee, A. B. (1979).Proc. Natl. Acad. Sci. U.S.A. (in press).
Sager, R., Cherington, P. V., Smith, B. L., and Pardee, A. B. (1979).Fed Proc. 38:1360A.
Howell, N., and Sager, R. (1979).Somat. Cell Genet. 5:129–143.
Ruddle, F., and Creagan, R. (1975).Annu. Rev. Genet. 9:407–486.
Handmaker, S. D. (1973).Annu. Rev. Microbiol. 27:189–204.
Schall, D., and Rechsteiner, M. (1978).Somat. Cell Genet. 4:661–676.
Minna, J., and Coon, H. (1974).Nature 252:401–404.
Engel, E., McGee, B. J., and Harris, H. (1969).Nature 223:152–155.
Yoshida, M. C., and Sasaki, M. (1978).Somat. Cell Genet. 4:437–450.
Sager, R., and Kitchin, R. (1975).Science 189:426–433.
Howell, N., and Sager, R. (1978).Proc. Natl. Acad. Sci. U.S.A. 75:2358–2362.
Author information
Authors and Affiliations
Additional information
Part II of this series was published inProc. Natl. Acad. Sci. U.S.A. 75(5):2358–2362 (1978); Part III was published inSomat. Cell Genet. 5:129–143 (1979).
Rights and permissions
About this article
Cite this article
Sager, R., Kovac, P.E. Genetic analysis of tumorigenesis: IV. chromosome reduction and marker segregation in progeny clones from Chinese hamster cell hybrids. Somat Cell Mol Genet 5, 491–502 (1979). https://doi.org/10.1007/BF01538883
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF01538883