Skip to main content
SpringerLink
Log in

Codominant translational mutants of Chinese hamster ovary cells selected with diphtheria toxin

  • Published:
Somatic Cell Genetics

Abstract

Diphtheria toxin-resistance markers in two translational mutants, CH-RE1.22c, possessing no toxin-sensitive EF-2 (class IIa), and CH-RE1.32, with 50% toxin-sensitive and 50% toxin-resistant EF-2 (class IIb), behaved codominantly in somatic cell hybrids. There was no complementation in hybrids formed between the two resistant mutants. The mutant parents and their hybrids, except those formed by fusion of CH-RE1.32 and wild-type cells, grew in the presence of toxin. To explain these results we suggest that CHO-K1 cells possess two functional copies of the gene for EF-2 and that CH-RE1.22c and CH-RE1.32 represent the homozygous (R/R) and heterozygous (R/S) states of resistance at the EF-2 gene locus. The failure of hybrids formed between CH-RE1.32 and wild-type cells to grow in toxin is a gene dosage effect. Codominant class IIa translational resistance is a selectable marker for the isolation of hybrids. It can be combined with a second, recessive, marker to provide a cell which is a “universal hybridizer” (10).

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Literature cited

  1. Moehring, J. M., and Moehring, T. J. (1979).Somat. Cell Genet. 5:453–468.

    PubMed  Google Scholar 

  2. Littlefield, J. W. (1964).Science 145:709–710.

    PubMed  Google Scholar 

  3. Sun, N. C., Chu, E. H. Y., and Chang, C. C. (1973).Mammal. Chromosome Newsl. 14:26–28.

    Google Scholar 

  4. Davidson, R. L., and Gerald, P. S. (1976).Somat. Cell Genet. 2:165–176.

    PubMed  Google Scholar 

  5. Worton, R. G. (1978).Cytogenet. Cell Genet. 21:105–110.

    PubMed  Google Scholar 

  6. Deaven, L. L., and Petersen, D. F. (1973).Chromosoma 41:129–144.

    PubMed  Google Scholar 

  7. Worton, R. G., Ho, C. C., and Duff, C. (1977).Somat. Cell Genet. 3:27–45.

    PubMed  Google Scholar 

  8. Siminovitch, L. (1976).Cell 7:1–11.

    PubMed  Google Scholar 

  9. Gupta, R. S., and Siminovitch, L. (1978).Somat. Cell Genet. 4:553–571.

    PubMed  Google Scholar 

  10. Corsaro, C. M., and Migeon, B. R. (1978).Somat. Cell Genet. 4:531–540.

    PubMed  Google Scholar 

  11. Robinson, E. A., Henrikson, O., and Maxwell, E. S. (1974).J. Biol. Chem. 249:5088–5093.

    PubMed  Google Scholar 

  12. Collier, R. J. (1975).Bacteriol. Rev. 39:54–85.

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Medical Microbiology, University of Vermont College of Medicine, 05405, Burlington, Vermont

    T. J. Moehring, D. E. Danley & J. M. Moehring

Authors
  1. T. J. Moehring
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. D. E. Danley
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. J. M. Moehring
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Moehring, T.J., Danley, D.E. & Moehring, J.M. Codominant translational mutants of Chinese hamster ovary cells selected with diphtheria toxin. Somat Cell Mol Genet 5, 469–480 (1979). https://doi.org/10.1007/BF01538881

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01538881

Keywords

Search

Navigation