Skip to main content
SpringerLink
Log in

Phenotypic expression of malignancy in hybrid and cybrid mouse cells

  • Published:
Somatic Cell Genetics

Abstract

This study has been directed toward the effect of cytoplasmic transfer on the expression of marker properties in hybrid cell systems. Conventional hybrids between two nucleated cells were constructed between tumorigenic and nontumorigenic cells. Cytoplasmic hybrids, or cybrids, were constructed between enucleated chloramphenicol-resistant (CAPR) donor cells (cytoplasts) and nucleated recipient cells. Clear-cut evidence for the cytoplasmic transmission of CAP resistance was obtained. Although cytoplasmic transfer had no effect on tumorigenicity or growth in soft agar, preliminary evidence was found that saturation density of the recipient cells could be altered by cytoplasmic addition in cybrids.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

Literature cited

  1. Barski, G., and Belehradek, J. (1974). InDifferentiation and Control of Malignancy of Tumor Cells, (eds.) Nakahara, W., Ono, T., and Sugano, H. (University Park Press, Baltimore), pp. 419–441.

    Google Scholar 

  2. Harris, H., (1971).Proc. R. Soc. London, Ser. B 179:1–20.

    Google Scholar 

  3. Harris, H., Miller, O. J., Klein, G., Worst, P., and Tachibana, T. (1969).Nature 223:363–368.

    PubMed  Google Scholar 

  4. Miller, R. A., and Ruddle, F. (1976).Cell 9:44–55.

    Google Scholar 

  5. Carter, S. B. (1967).Nature 213:261–264.

    PubMed  Google Scholar 

  6. Bunn, C. L., Wallace, D. C., and Eisenstadt, J. M. (1974).Proc. Natl. Acad. Sci. U.S.A. 71:1681–1685.

    PubMed  Google Scholar 

  7. Beisson, J., Sainsard, A., Adoutte, A., Beale, G. H., Knowles, J., and Tait, A., (1974).Genetics 78:403–413.

    PubMed  Google Scholar 

  8. Medina, D., and DeOme, K. B. (1970).Cancer Res. 30:1055–1059.

    PubMed  Google Scholar 

  9. Prescott, D. M., Meyerson, D., and Wallace, J. (1972).Exp. Cell Res. 71:480–485.

    PubMed  Google Scholar 

  10. Davidson, R. L., O'Malley, K. A., and Wheeler, T. B. (1976).Somat. Cell Genet. 2:271–280.

    PubMed  Google Scholar 

  11. Puck, T. T., Marcus, P. I., and Cieciura, S. J. (1956).J. Exp. Med. 103:273–290.

    PubMed  Google Scholar 

  12. Wallace, D. C., Bunn, C. L., and Eisenstadt, J. M. (1975).J. Cell Biol. 67:174–188.

    PubMed  Google Scholar 

  13. Jha, K. K., and Ozer, H. L. (1976).Somat. Cell Genet. 2:215–223.

    PubMed  Google Scholar 

  14. Deaven, L. L., and Peterson, D. F. (1973).Chromosoma 41:129–144.

    PubMed  Google Scholar 

  15. Harris, M. (1975).J. Cell. Physiol. 86:413–430.

    PubMed  Google Scholar 

  16. MacPherson, I., and Montagnier, L. (1964).Virology 23:291–294.

    PubMed  Google Scholar 

  17. Bartholomew, J. C., Yokata, H., and Ross, P. (1976).J. Cell. Physiol. 88:277–286.

    PubMed  Google Scholar 

  18. Clements, G. B., Fenyo, E. M., and Klein, G. (1976).Proc. Natl. Acad. Sci. U.S.A. 73:2004–2007.

    PubMed  Google Scholar 

  19. Croce, C. M., Aden, D., and Koprowski, H. (1975).Science 190:1200–1202.

    PubMed  Google Scholar 

  20. Goldenberg, D. M., Pavia, R. A., and Tsao, M. D. (1974).Nature 250:649–651.

    PubMed  Google Scholar 

  21. Aviles, D., Jami. J., Rousset, J., and Ritz, E. (1977).J. Natl. Cancer Inst. 58:1391–1399.

    PubMed  Google Scholar 

  22. Jonasson, J., and Harris, H. (1977).J. Cell Sci. 24:255–263.

    PubMed  Google Scholar 

Download references

Author information

Author notes

  1. Michael L. Ziegler

    Present address: Division of Human Genetics, Children's Hospital Medical Center, 02115, Boston, Massachusetts

Authors and Affiliations

  1. Department of Zoology and Cancer Research Laboratory, University of California, 94720, Berkeley, California

    Michael L. Ziegler

Authors
  1. Michael L. Ziegler
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ziegler, M.L. Phenotypic expression of malignancy in hybrid and cybrid mouse cells. Somat Cell Mol Genet 4, 477–489 (1978). https://doi.org/10.1007/BF01538868

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01538868

Keywords

Search

Navigation