Skip to main content
SpringerLink
Log in

Induced segregation of human syntenic genes by 5-bromodeoxyuridine + near-visible light

  • Published:
Somatic Cell Genetics

Abstract

Chromosome-breaking agents have been used in two different procedures for promoting segregation of syntenic genes on human chromosome 12. In method A, a human-Chinese hamster cell hybrid containing the single human chromosome 12 was treated either with 5-bromodeoxyuridine BrdU + near-visible light or with X-rays. In method B, normal human fibroblasts were treated with BrdU + near-visible light followed by their fusion with a Chinese hamster glycine-requiring cell mutant CHO- K1/gly−A. Since the human complementing gene for serine hydroxymethyltransferase, an enzyme deficient in gly−A, lies on human chromosome 12, only those hybrids retaining that chromosome can survive the glycine-free medium. Clones isolated from both procedures were analyzed for the loss or retention of four other syntenic genes on chromosome 12, TPI, GAPD, LDHB, and PepB. The results demonstrate that method B is much more effective in generating clones with extensive marker losses. In addition, the segregation pattern and frequency obtained in this study provided information on the linear order of TPI and GAPD on chromosome 12.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Literature cited

  1. Puck, T. T. (1972).The Mammalian Cell as a Microorganism (Holden-Day, San Francisco).

    Google Scholar 

  2. McKusick, V. A., and Ruddle, F. H. (1977).Science 196:390–405.

    PubMed  Google Scholar 

  3. Ruddle, F. H., and Creagan, R. P. (1975).Annu. Rev. Genet. 9:407–486.

    PubMed  Google Scholar 

  4. Burgerhout, W., Van Someren, H., and Bootsma, D. (1973).Humangenetik 20:159–162.

    PubMed  Google Scholar 

  5. Burgerhout, W., Leupe-De Smit, S., and Jongsma, A. P. M. (1977).Cytogenet. Cell Genet. 18:267–283.

    PubMed  Google Scholar 

  6. Goss, S. J., and Harris, H. (1975).Nature 255:680–684.

    PubMed  Google Scholar 

  7. Goss, S. J., and Harris, H. (1977).J. Cell Sci. 25:17–37.

    PubMed  Google Scholar 

  8. Goss, S. J., and Harris, H. (1977).J. Cell Sci. 25:39–58.

    PubMed  Google Scholar 

  9. Puck, T. T., and Kao, F. T. (1967).Proc. Natl. Acad. Sci. U.S.A. 58:1227–1234.

    PubMed  Google Scholar 

  10. Kao, F. T., Chasin, L., and Puck, T. T. (1969).Proc. Natl. Acad. Sci. U.S.A. 64:1284–1291.

    PubMed  Google Scholar 

  11. Jones, C., Wuthier, P., Kao, F. T., and Puck, T. T. (1972).J. Cell. Physiol. 80:291–298.

    PubMed  Google Scholar 

  12. Kao, F. T., Jones, C., and Puck, T. T. (1976).Proc. Natl. Acad. Sci. U.S.A. 73:193–197.

    PubMed  Google Scholar 

  13. Kao, F. T., and Puck, T. T. (1968).Proc. Natl. Acad. Sci. U.S.A. 60:1275–1281.

    PubMed  Google Scholar 

  14. Kao, F. T., and Puck, T. T. (1974). InMethods in Cell Biology, Vol. 8, (ed.) Prescott, D. M. (Academic Press, New York), pp. 23–29.

    Google Scholar 

  15. Kao, F. T., and Puck, T. T. (1972).J. Cell. Physiol. 80:41–50.

    PubMed  Google Scholar 

  16. Ham, R. G. (1965).Proc. Natl. Acad. Sci. U.S.A. 53:288–293.

    PubMed  Google Scholar 

  17. Ham, R. G., and Puck, T. T. (1962). InMethods in Enzymology, Vol. 5, (eds.) Colowick, S. P., and Kaplan, N. O. (Academic Press, New York), pp. 90–119.

    Google Scholar 

  18. Kao, F. T., and Puck, T. T. (1969).J. Cell. Physiol. 74:245–258.

    PubMed  Google Scholar 

  19. Kao, F. T., Johnson, R. T., and Puck, T. T. (1969).Science 164:312–314.

    PubMed  Google Scholar 

  20. Baker, R. M. (1976). InBiogenesis and Turnover of Membrane Macromolecules, (ed.) Cook, J. S. (Raven Press, New York), pp. 93–103.

    Google Scholar 

  21. Van Someren, H., Van Henegcuwen, H. B., Los, W., Wurzer-Figurelli, E., Doppert, B., Vervloet, M., and MeeraKhan, P. (1974).Humangenetik 25:189–201.

    PubMed  Google Scholar 

  22. Bruns, G. A. P., and Gerald, P. S. (1976).Science 192:54–56.

    PubMed  Google Scholar 

  23. Kao, F. T., Jones, C., and Puck, T. T. (1977).Somat. Cell Genet. 3:421–429.

    PubMed  Google Scholar 

  24. Harris, J. I., and Perham, R. N. (1965).J. Mol. Biol. 13:876–884.

    Google Scholar 

  25. Rethoré, M. O., Junien, Cl., Malpuech, G., Baccichetti, C., Tenconi, R., Kaplan, H. Cl., Romeuf, J. de, and Lejeune, J. (1976).Ann. Genet. 19:140–142.

    PubMed  Google Scholar 

  26. Rethoré, M. O., Kaplan, J. Cl., Junien, C., and Lejuene, J. (1977).Hum. Genet. 36:235–237.

    PubMed  Google Scholar 

  27. Buck, D. W., Goss, S. J., and Bodmer, W. F. (1976) In Baltimore Conference (1975): Third International Workshop on Human Gene Mapping.Birth Defects: Original Article Series, Vol. 12, No. 7, 1976 (The National Foundation, New York) pp. 99–100.

    Google Scholar 

  28. Kao, F. T., Jones, C., Law, M. Liao, and Puck, T. T. (1978) Winnipeg Conference (1977): Fourth International Workshop on Human Gene Mapping. In press.

Download references

Author information

Authors and Affiliations

  1. Eleanor Roosevelt Institute for Cancer Research, and Department of Biophysics and Genetics, University of Colorado Medical Center, 80262, Denver, Colorado

    Martha Liao Law & Fa -Ten Kao

Authors
  1. Martha Liao Law
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Fa -Ten Kao
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

This paper is No. 24 in the series entitled, “Genetics of Somatic Mammalian Cells.” The preceding paper is Ref. 23.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Law, M.L., Kao, F.T. Induced segregation of human syntenic genes by 5-bromodeoxyuridine + near-visible light. Somat Cell Mol Genet 4, 465–476 (1978). https://doi.org/10.1007/BF01538867

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01538867

Keywords

Search

Navigation