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Concentration-dependent mutation of diploid human lymphoblasts by methylnitronitrosoguanidine: The importance of phenotypic lag

Somatic Cell Genetics volume 2, pages 325–330 (1976)Cite this article

Abstract

The mutation of diploid human lymphoblasts by methylnitronitrosoguanidine (MNNG) was measured over the range of 0–45 ng of MNNG/ml of medium. We found a 12-day lag in the phenotypic expression of 6-thioguanine resistance; the occurrence of this lag was independent of MNNG concentration. We hypothesize that the unexpectedly long lag period reflects a requirement for the loss of previously existing molecules of the enzyme hypoxanthine-guanine phosphoribosyltransferase (HGPRT) after mutation at the HGPRT locus.

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Affiliations

  1. Toxicology Group, Department of Nutrition and Food Science, Massachusetts Institute of Technology, 02139, Cambridge, Massachusetts

    B. W. Penman & W. G. Thilly

Authors
  1. B. W. Penman
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  2. W. G. Thilly
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Penman, B.W., Thilly, W.G. Concentration-dependent mutation of diploid human lymphoblasts by methylnitronitrosoguanidine: The importance of phenotypic lag. Somat Cell Mol Genet 2, 325–330 (1976). https://doi.org/10.1007/BF01538837

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  • DOI: https://doi.org/10.1007/BF01538837

Keywords

  • Phenotypic Expression
  • Human Lymphoblast
  • MNNG Concentration
  • HGPRT Locus