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Concentration-dependent mutation of diploid human lymphoblasts by methylnitronitrosoguanidine: The importance of phenotypic lag

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Somatic Cell Genetics

Abstract

The mutation of diploid human lymphoblasts by methylnitronitrosoguanidine (MNNG) was measured over the range of 0–45 ng of MNNG/ml of medium. We found a 12-day lag in the phenotypic expression of 6-thioguanine resistance; the occurrence of this lag was independent of MNNG concentration. We hypothesize that the unexpectedly long lag period reflects a requirement for the loss of previously existing molecules of the enzyme hypoxanthine-guanine phosphoribosyltransferase (HGPRT) after mutation at the HGPRT locus.

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Authors and Affiliations

  1. Toxicology Group, Department of Nutrition and Food Science, Massachusetts Institute of Technology, 02139, Cambridge, Massachusetts

    B. W. Penman & W. G. Thilly

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  1. B. W. Penman
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  2. W. G. Thilly
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Penman, B.W., Thilly, W.G. Concentration-dependent mutation of diploid human lymphoblasts by methylnitronitrosoguanidine: The importance of phenotypic lag. Somat Cell Mol Genet 2, 325–330 (1976). https://doi.org/10.1007/BF01538837

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  • DOI: https://doi.org/10.1007/BF01538837

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