Abstract
Fusion of ASL-1 cells, a murine leukemia forming thymus leukemia (TL) antigens, with LM(TK)− cells, a TL(−) murine cell line, resulted in a stable hybrid forming TL antigens. The hybrids failed to undergo modulation, the reversible disappearance of TL antigens from the surfaces of the cells, stimulated by TL antiserum. Unlike ASL-1 cells, the rate of disappearance of the antigens from modulation negative hybrid cells was unaffected by TL antiserum. The t1/2 of TL antigens of the hybrid was approximately 30 h. The t1/2 of TL antigens of ASL-1 cells was 10 h in the presence of TL antiserum, 18 h in the absence of TL antiserum. The rate of metabolism of a putative tumorassociated antigen of ASL-1 cells formed by the hybrid was unaffected by exposure to specific antiserum, as was the metabolism of H-2 antigens formed by the cell types.
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Liang, W., Cohen, E.P. The effect of specific antiserum on the metabolism of three membrane-associated antigens of ASL-1 × LM(TK)−hybrid cells. Somat Cell Mol Genet 2, 291–307 (1976). https://doi.org/10.1007/BF01538835
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DOI: https://doi.org/10.1007/BF01538835