Summary
Hepatitis C virus (HCV) is currently classified into 6 major types, HCV-1 through -6, each of which can be further divided into a few subtypes, e.g., HCV-1a, -1b, -1c, etc., on the basis of sequence variation of the viral genome. The core and E1 envelope regions of HCV genome were amplified from sera of Indonesian patients using reverse transcription-polymerase chain reaction. Sequence analysis of both core and E1 regions followed by molecular evolutionary phylogenetic analysis identified a novel sequence variant of HCV-1 (Td-6). Antibodies in the serum from which Td-6 was isolated reacted only marginally to the core protein of HCV-J, a representative strain of HCV-1b, despite strong antibody response against a mixture of the core, NS3 and NS4 proteins of HCV-1a. The possible mechanism for the diminished reactivity of the antibodies in the serum to the core protein of HCV-J is discussed.
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The nucleotide sequence data reported in this paper will appear in the DDBJ/EMBL/GenBank Nucleotide Sequence Databases with the accession numbers D13729 to D13736, and D14669 to D14671.
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Hotta, H., Doi, H., Hayashi, T. et al. Analysis of the core and E1 envelope region sequences of a novel variant of hepatitis C virus obtained in Indonesia. Archives of Virology 136, 53–62 (1994). https://doi.org/10.1007/BF01538816
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DOI: https://doi.org/10.1007/BF01538816