Archives of Virology

, Volume 136, Issue 1–2, pp 19–34 | Cite as

Early interaction of feline calicivirus with cells in culture

  • L. C. Kreutz
  • B. S. Seal
  • W. L. Mengeling
Original Papers


The kinetics and biochemical properties of feline calicivirus (FCV) attachment to Crandell-Reese feline kidney cells were determined. Maximum binding was observed at pH 6.5. Cells in suspension at 4°C bound virus more efficiently than cells in monolayers at 4°C or 37°C. High initial binding rate was observed in monolayers or cells in suspension and proceeded to a maximum at 90 min, although half maximal binding was observed as early as 15 min. Binding was specific and competitively blocked by serotypically homologous or heterologous FCV as well as by San Miguel sea lion virus. Treatment of cells with proteases increased FCV binding, whereas phospholipase had no effect on virus attachment. Conversely, cells treated with neuraminidase followed by O-glycanase treatment showed a decreased binding ability. Cells of feline origin bound FCV very efficiently, and non-permissive cells showed a poor binding ability. Following transfection of viral RNA, infectious virus could be recovered from all non-permissive cells, except from Madin-Darby canine kidney cells. These results suggest that FCV binds to a receptor in which carbohydrates may be an important component and that FCV replication in nonpermissive cells is primarily restricted by the absence of appropriate receptors on the cell surface.


Kidney Cell Binding Ability Binding Rate Virus Attachment Initial Binding 
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  1. 1.
    Abraham G, Colonno RJ (1984) Many rhinovirus serotypes share the same cellular receptor. J Virol 51: 340–345PubMedGoogle Scholar
  2. 2.
    Bachrach HL, Trautman R, Breese, Jr SS (1964) Chemical and physical properties of virtually pure foot- and -mouth disease virus. Am J Vet Res 25: 333–342PubMedGoogle Scholar
  3. 3.
    Bass DM, BAylor MR, Chen C, Mackow EM, Bremont M, Greenberg HB (1992) Liposome mediated transfection of intact viral particles reveals that plasma membrane penetration determines permissivity of tissue culture cells to rotavirus. J Clin Invest 90: 2313–2320PubMedGoogle Scholar
  4. 4.
    Bass DM, Mackow ER, Greenberg HB (1991) Identification and partial characterization of a rhesus rotavirus binding glycoprotein on Murine erythrocytes. Virology 183: 602–610PubMedGoogle Scholar
  5. 5.
    Baxt B, Bachrach HL (1980) Early interactions of foot-and-mouth disease virus with cultured cells. Virology 104: 42–55PubMedGoogle Scholar
  6. 6.
    Burroughs JN, Brown F (1978) Presence of a covalently linked protein on calicivirus RNA. J Gen Virol 41: 443–446PubMedGoogle Scholar
  7. 7.
    Carter MJ, Milto ID, Meanger J, Bennett M, Gaskell RM, Turner PC (1992) The complete nucleotide sequence of feline calicivirus. Virology 190: 443–448PubMedGoogle Scholar
  8. 8.
    Clayson ET, Compans RW (1989) Characterization of simian virus 40 receptor moieties on the surface of Vero C1008 cells. J Virol 63: 1095–1100PubMedGoogle Scholar
  9. 9.
    Courderc T, Barzu T, Horaud F, Crainic R (1990) Poliovirus permissivity and specific receptor expression on human endothelial cells. Virology 174: 95–102PubMedGoogle Scholar
  10. 10.
    Crandell RA (1988) Isolation and characterization of caliciviruses from dogs with vesicular genital disease. Arch Virol 98: 65–71PubMedGoogle Scholar
  11. 11.
    Crandell RA, Fabricant CA, Nelson-Rees WA (1973) Development, characterization, and viral susceptibility of a feline (Felis catus) renal cell line (CRFK). In Vitro 9: 176–185PubMedGoogle Scholar
  12. 12.
    Crandell RA, Neimann WH, Ganaway JR, Maurer FD (1960) Isolation of cytopathic agents from the nasopharyngeal region of the domestic cat. Virology 10: 283–285PubMedGoogle Scholar
  13. 13.
    Cubitt WD, Barrett ADT (1984) Propagation of human candidate calicivirus in cell culture. J Gen Virol 65: 1123–1126PubMedGoogle Scholar
  14. 14.
    Cubitt WD, Barrett ADT (1985) Propagation and preliminary characterization of a chicken candidate calicivirus. J Gen Virol 66: 1431–1438PubMedGoogle Scholar
  15. 15.
    Dalgleish AG, Beverley PCL, Claphan PR, Crawford DH, Greave MF, Weiss RA (1984) The CD4 (T4) antigen is an essential component of the receptor for the AIDS retrovirus. Nature 312: 763–767PubMedGoogle Scholar
  16. 16.
    Dimmock NJ (1982) Initial stages in infection with animal viruses. J Gen Virol 59: 1–22PubMedGoogle Scholar
  17. 17.
    Dveksler Gs, Pensiero MN, Cardellichio CB, Williams RK, Jiang G, Holmes KV, Dieffenbach CW (1991) Cloning of the mouse hepatitis virus (MHV) receptor: expression in human and hamster cell lines confers susceptibility to MHV. J Virol 65: 6881–6891PubMedGoogle Scholar
  18. 18.
    Fastier LB (1957) A new feline virus isolated in tissue culture. Am J Vet Res 18: 382–389PubMedGoogle Scholar
  19. 19.
    Fodiatis C, Kilpatrick DR, Lipton HL (1991) Comparison of the binding characteristics to BHK-1 cells of viruses representing the two Theiler's virus neurovirulence groups. Virology 182: 365–370PubMedGoogle Scholar
  20. 20.
    Fried H, Cahan LD, Paulson JC (1981) Polyoma virus recognize specific sialyloligo-saccharide receptors on host cells. Virology 109: 188–192PubMedGoogle Scholar
  21. 21.
    Herrler G, Klenk H (1987) The surface receptor is a major determinant of the cell tropism of influenza virus. Virology 159: 102–108PubMedGoogle Scholar
  22. 22.
    Johnson RH (1965) Feline panleucopaenia. I. Identification of a virus associated with the syndrome. Res Vet Sci 6: 466–471PubMedGoogle Scholar
  23. 23.
    Innes CL, Smith PB, Langerbach R, Tindal KR, Boone LR (1990) Cationic liposome (Lipofectin) mediated retroviral infection in the absence of specific receptors. J Virol 64: 957–961PubMedGoogle Scholar
  24. 24.
    Lonberg-Holm K, Crowell RL, Philipson L (1976) Unrelated animal viruses share receptors. Nature 259: 679–681PubMedGoogle Scholar
  25. 25.
    Lonberg-Holm K, Korant BD (1972) Early interaction of rhinoviruses with host cells. J Virol 9: 29–40PubMedGoogle Scholar
  26. 26.
    Lonberg-Holm K, Philipson L (1974) Early interaction between animal viruses and cells. Monogr Virol 9: 1–48PubMedGoogle Scholar
  27. 27.
    Lonberg-Holm K, Whiteley NM (1976) Physical and metabolic requirements for early interaction of poliovirus and human rhinovirus with Hela cells. J Virol 19: 857–870PubMedGoogle Scholar
  28. 28.
    Love DN (1976) Feline calicivirus: purification of virus and extraction and characterization of its ribonucleic acid. Cornell Vet 66: 498–512PubMedGoogle Scholar
  29. 29.
    Mak TW, O'Callaghan J, Colter JS (1970) Studies of the early events of the replicative cycle of three variants of mengo encephalomyelitis virus in mouse fibroblast cells. Virology 42: 1087–1096PubMedGoogle Scholar
  30. 30.
    Malone RW, Felgner PL, Verma IM (1989) Cationic liposo-mediated RNA transfection. Proc Natl Acad Sci USA 86: 6077–6081PubMedGoogle Scholar
  31. 31.
    March M, Heleniust A (1989) Virus entry into animal cells. Adv Virus Res 36: 107–151PubMedGoogle Scholar
  32. 32.
    McClintock PR, Billups LC, Notkins AB (1980) Receptors for encephalomyocarditis virus on murine and human cells. Virology 106: 261–272PubMedGoogle Scholar
  33. 33.
    Nowlin DM, Cooper NR, Compton T (1991) Expression of a human cytomegalovirus receptor correlates with infectibility of cells. J Virol 65: 3114–3121PubMedGoogle Scholar
  34. 34.
    Ohlinger VH, Haas G, Weiland F, Thiel HU (1990) Identification and characterization of a virus causing rabbit hemorrhagic disease. J Virol 64: 3331–3336PubMedGoogle Scholar
  35. 35.
    Parra F, Pietro M (1990) Purification and characterization of a calicivirus as the causative agent of lethal hemorrhagic disease in rabbits. J Virol 64: 4013–4015PubMedGoogle Scholar
  36. 36.
    Schaffer FL (1979) Caliciviruses, In: Frankel-Conrat H, Wagner R (eds) Comparative virology, vol 14. Plenum Press, New York, pp 249–284Google Scholar
  37. 37.
    Schaffer FL, Burroughs JN, Scott F (1980) Caliciviridae. Intervirology 14: 1–6PubMedGoogle Scholar
  38. 38.
    Schaffer FL, Soergel ME (1976) Single major polypeptide of a calicivirus: characterization by polyacrylamide gel electrophoresis and stabilization of virions by cross-linking with dimethyl suberimidate. J Virol 19: 925–931PubMedGoogle Scholar
  39. 39.
    Seal BS, Whetstone CA, Zamb TJ, Bello LJ, Lawrence WC (1992) Relationship of bovine herpesvirus 1 immediate-early, early, and late gene expression to host cellular gene transcription. Virology 188: 152–159PubMedGoogle Scholar
  40. 40.
    Smith AW, Skilling DE, Dardir AH, Latham AB (1980) Calicivirus pathogenic for swine: a new serotype isolated from Opaleye Girella nigricans, an ocean fish. Science 209: 940–941PubMedGoogle Scholar
  41. 41.
    Smith AW, Skilling DE, Ensley PK, Benirschke K, Lester TK (1983) Calicivirus isolation and persistence in a Pygmy chimpanzee (pan paniscus). Science 221: 79–81PubMedGoogle Scholar
  42. 42.
    Takeuchi Y, Vile RG, Simpson G, O'Hara B, Collins MKL, Weiss RA (1992) Feline leukemia virus subgroup B uses the same cell surface receptor as Gibbon ape leukemia virus. J Virol 66: 1219–1222PubMedGoogle Scholar
  43. 43.
    Wunner WH, Reagan KJ, Koprowski H (1984) Characterization of saturable binding sites for rabies virus. J Virol 50: 691–697PubMedGoogle Scholar

Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • L. C. Kreutz
    • 1
  • B. S. Seal
    • 1
  • W. L. Mengeling
    • 1
  1. 1.Virology Swine Research Unit, National Animal Disease CenterUSDA, Agricultural Research ServiceAmesUSA

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