Abstract
Ribonucleotide reductase activity and deoxyribonucleoside triphosphate (dNTP) pools were examined in several Syrian hamster melanoma cell mutants which are resistant to hydroxyurea (HU), an inhibitor of ribonucleotide reductase, and which also show increased resistance to bromodeoxyuridine (BrdU) mutagenesis. For most of the mutants, resistance to HU and BrdU mutagenesis is associated with increased levels of ribonucleotide reductase activity. No evidence was found for qualitative alterations in the ribonucleotide reductase in the mutant cells. The dNTP pools in the mutants are somewhat resistant to the perturbations produced in wild-type cells by the addition of BrdU, although significant perturbations can be produced in the mutants by higher concentrations of BrdU. The decrease in BrdU-induced nucleotide pool perturbations may account for the resistance of the mutants to BrdU mutagenesis.
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Kaufman, E.R., and Davidson, R.L. (1978).Proc. Natl. Acad. Sci. U.S.A. 75:4982–4986.
Davidson, R.L., and Kaufman, E.R. (1978).Nature 276:722–723.
Kaufman, E.R., and Davidson, R.L. (1979).Somat. Cell Genet. 5:553–563.
Ashman, C.R., and Davidson, R.L. (1981).Mol. Cell. Biol. 1:254–260.
Reichard, P., Cannellakis, Z.N., and Cannellakis, E.S. (1961).J. Biol. Chem. 236:2514–2519.
Davidson, R.L., and Kaufman, E.R. (1979).Somat. Cell Genet. 5:873–885.
Kaufman, E.R., and Davidson, R.L. (1977).Exp. Cell Res. 107:15–24.
Lewis, W., McNaughton, D., Goh, S. LeJohn, H., and Wright, J. (1977).J. Cell. Physiol. 93:345–352.
Cory, J.G. (1973).Cancer Res. 33:993–998.
Steeper, J.R., and Stewart, C.D. (1970).Anal. Biochem. 34:123–130.
Lowry, O., Rosebrough, N., Farr, A., and Randall, R. (1951).J. Biol. Chem. 193:265–275.
Reddy, G.P.V., and Pardee, A.B. (1980).Proc. Natl. Acad. Sci. U.S.A. 77:3312–3316.
Miller, M.R., Castellot, J.J., Jr., and Pardee, A.B. (1978).Biochemistry 17:1073–1080.
Lindbergh, U., and Skoog, L. (1970).Anal. Biochem. 34:152–160.
Skoog, L. (1970).Eur. J. Biochem. 17:202–208.
Bick, M.D. (1977).Anal. Biochem. 78:582–585.
Baker, R.M., Brunette, D.M., Mankowitz, R., Thompson, L.H., Whitmore, G.F., Siminovitch, L. and Till, J.E. (1974).Cell 1:9–21.
Moore, E.C., and Hurlbert, R.B. (1966).J. Biol. Chem. 241:4802–4809.
Meuth, M., and Green, H. (1974).Cell 2:109–112.
Lewis, W., and Wright, J. (1974).Biochem. Biophys. Res. Commun. 71:128–135.
Lewis, W., and Wright, J. (1979).Somat. Cell Genet. 5:83–96.
Kuzik, B.A., and Wright, J.A. (1980).Biochem. Genet. 18:311–331.
Koropatnick, S.E., and Wright, J.A. (1980).Enzyme 25:220–227.
Meuth, M., Aufreiter, E., and Reichard, P. (1976).Eur. J. Biochem. 71:39–43.
Ullman, B., Gudas, L.J., Clift, S., and Martin, D.W. (1979).Proc. Natl. Acad. Sci. U.S.A. 76:1074–1078.
Peterson, A.R., Landolph, J.R., Peterson, C., and Heidelberger, C. (1978).Nature 276:508–510.
Meuth, M. (1981).Somat. Cell Genet. 7:89–102.
Meuth, M., L'Heureux-Huard, N., and Trudel, M. (1979).Proc. Natl. Acad. Sci. U.S.A. 76:6505–6509.
Kunkel, T.A., and Loeb, L.A. (1979).J. Biol. Chem. 254:5718–5725.
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Ashman, C.R., Reddy, G.P.V. & Davidson, R.L. Bromodeoxyuridine mutagenesis, ribonucleotide reductase activity, and deoxyribonucleotide pools in hydroxyurea-resistant mutants. Somat Cell Mol Genet 7, 751–768 (1981). https://doi.org/10.1007/BF01538762
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DOI: https://doi.org/10.1007/BF01538762