Abstract
Polyethylene glycol (PEG) is known to promote fusion of plant protoplasts. Various adaptations of this treatment to mammalian, including human, cell cultures are reported here. PEG is very effective in producing hybrids capable of indefinite multiplication even in cases, such as early passage human skin fibroblasts and lymphocytes, known to be highly recalcitrant to other treatments.
Literature cited
Harris, H., and Watkins, J.F. (1965).Nature (London) 205:640–646.
Yerganian, G., and Nell, M.B. (1966).Proc. Natl. Acad. Sci. U.S.A. 55:1066–1073.
Lucy, J.A., Ahkong, Q.F., Cramp, F.C., Fisher, D., and Howell, J.I. (1971).Biochem. J. 124:46–47.
Croce, C.M., Koprowski, H., and Eagle, H. (1972).Proc. Natl. Acad. Sci. U.S.A. 69:1953–56.
Ahkong, Q.F., Cramp, F.C., Fisher, D., Howell, J.I., and Lucy, J.A. (1972).J. Cell Sci. 10:769–787.
Cramp, F.C., and Lucy, J.A. (1974).Exp. Cell Res. 87:107–110.
Cocking, E.C. (1973).Colloq. Int. C.N.R.S. 212:327–342.
Kao, K.N., and Michayluk, M.R. (1974).Planta (Berlin) 115:355–367.
Constabel, F., and Kao, K.N. (1974).Can. J. Bot. 52:1603–1606.
Bonnett, H.T., and Eriksson, T. (1974).Planta (Berlin) 120:71–79.
Pontecorvo, G. (1971).Nature (London) 230:367–369.
Pontecorvo, G. (1974). In Davidson, R.L., and de la Cruz, F. (eds.),Somatic Cell Hybridization, New York, pp. 65–69.
Littlefield, J. (1964).Science 145:709–710.
Ahkong, Q.F., Howell, J.I., Lucy, J.A., Safwat, F., Davey, M.R., and Cocking, E.C. (1975).Nature (London) 255:66–67.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Pontecorvo, G. Production of mammalian somatic cell hybrids by means of polyethylene glycol treatment. Somat Cell Mol Genet 1, 397–400 (1975). https://doi.org/10.1007/BF01538671
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF01538671