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Replicative potential of individual cell hybrids derived from young and old donor human skin fibroblasts

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Somatic Cell Genetics

Abstract

Neonatal, adult, and aged donor human skin fibroblast cell cultures have been characterized for the population doubling potential and in vitro-in vivo relationship. Hybrid cells derived from individual whole-cell fusions of replicating GRC 387 (aged) and CSC 303 (neonatal) cells demonstrated that an intermediate mode of replication between that of the two parental cell lines occurred; therefore the longevity of the aged fibroblast cells was enhanced. The GRC 387 cells have an extended post-replicative phase-out period in comparison with the CSC 303 cells, and the experimental hybrids demonstrated a 20–25 % increase of this period over that of the GRC 387 cells.

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Literature cited

  1. Norwood, T.H., Pendergrass, W.R., and Martin, G.M. (1974).J. Cell Biol. 64:551–556.

    Google Scholar 

  2. Wright, W.E., and Hayflick, L. (1975).Exp. Cell Res. 96:113–121.

    PubMed  Google Scholar 

  3. Yanishevsky, R., and Stein, G.H. (1977). InCellular Senescence and Somatic Cell Genetics, Vol. 2, (eds.) Nichols, W.W., and Murphy, D.G. (Plenum Press, New York), pp. 73–81.

    Google Scholar 

  4. Muggleton-Harris, A.L., and Hayflick, L. (1976).Exp. Cell Res. 103:321–330.

    PubMed  Google Scholar 

  5. Bunn, C.L., and Tarrant, G.M. (1979).J. Cell Biol. 83:441–446.

    Google Scholar 

  6. Muggleton-Harris, A.L., and Palumbo, M. (1979).Somat Cell Genet. 5:379–407.

    Google Scholar 

  7. Muggleton-Harris, A.L., and DeSimone, D.W. (1980).Somat. Cell. Genet. 6:689–698.

    PubMed  Google Scholar 

  8. Pereira-Smith, O.M. and Smith, J.R. (1981).Somat. Cell Genet. 7:411–421.

    PubMed  Google Scholar 

  9. Bunn, C.L., and Tarrant, G.M. (1980).Exp. Cell Res. 127(2):385–396.

    PubMed  Google Scholar 

  10. Hayflick, L. (1965).Exp. Cell Res. 37:614–636.

    PubMed  Google Scholar 

  11. Schneider, E.L., and Mitsui, Y. (1976).Proc. Natl. Acad. Sci. U.S.A. 73(10):3584–3588.

    PubMed  Google Scholar 

  12. Smith, J.R., Pereira-Smith, S.M., and Schneider, E.L. (1978).Proc. Natl. Acad. Sci. U.S.A. 75:1353–1356.

    PubMed  Google Scholar 

  13. LeGuilly, Y., Simon, M., Lenoir, P., and Gourel, M. (1973).Gerontology 19:303–313.

    Google Scholar 

  14. Hoehn, H., Bryant, E.M., and Martin, G. (1978).Cytogenet. Cell Genet. 21:282–295.

    PubMed  Google Scholar 

  15. Martin, G.M., Sprague, C.A., and Epstein, C.J. (1970).Lab. Invest. 23:86–92.

    PubMed  Google Scholar 

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Authors and Affiliations

  1. Life Sciences Department, Worcester Polytechnic Institute, 01609, Worcester, Massachusetts

    A. L. Muggleton-Harris & M. A. Aroian

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  1. A. L. Muggleton-Harris
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  2. M. A. Aroian
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Muggleton-Harris, A.L., Aroian, M.A. Replicative potential of individual cell hybrids derived from young and old donor human skin fibroblasts. Somat Cell Mol Genet 8, 41–50 (1982). https://doi.org/10.1007/BF01538649

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  • DOI: https://doi.org/10.1007/BF01538649

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