Abstract
Drosophila S3 line cells maintain a high basal level of acetylcholinesterase (AChE) activity, which is lost and then reinduced following exposure to the steroid, β-ecdysone. Electrophoretic studies indicate that the basal and reinduced AChE activities may be different isozymes. In contrast, MDR line cells have a low basal AChE activity which is induced to a modest level by hormone. In S3/MDR hybrids the high basal level phenotype is extinguished, and the hormone-induced AChE level is modest. MDER line cells, which continue to grow in the presence of β-ecdysone and are therefore termed resistant, show a low basal AChE level that is not further elevated by hormone. This phenotype and response also occurred in β-ecdysone-resistant S3/MDER hybrids.
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Courgeon, A.M. (1972).Nature (London), New Biol. 238:250.
Cherbas, P., Cherbas, L., and Williams, C.M. (1977).Science 197:275.
Berger, E., Ringler, R., and Alahiotis, S. (1978).Dev. Biol. 62:498.
Wyss, C., and Eppenberger, H.M. (1978).Experientia 34:961.
Wyss, C. (1980).Exp. Cell Res. 125:121.
Courgeon, A.M. (1972).Exp. Cell Res. 74:327.
Metakovskii, E.V., Cherdantseva, E. M., and Gvozdev, V. A. (1977).Mol. Biol. 11:158.
Berger, E. (1979).In Vitro 15:211.
Best-Belpomme, M., and Courgeon, A.M. (1977).FEBS Lett. 82:345.
Best-Belpomme, M., Courgeon, A.M., and Rambach, A. (1978).Proc. Natl. Acad. Sci. U.S.A. 75:6102.
Berger, E., Frank, M., and Abell, M.C. (1980). InInvertebrate Systems In Vitro, (eds.) Kurstak, E., Mararmorosch, M., and Dubendorfer, A., (Elsevier, Amsterdam), p. 195.
Maroy, P., Dennis, R., Beckers, C., Sage, B.A., and O'Connor, J.D. (1978).Proc. Natl. Acad. Sci. U.S.A. 75:6035.
Wyss, C. (1979).Somat. Cell Genet. 5:29.
Schneider, I. (1972).J. Embryol. Exp. Morphol. 27:353.
Echalier, G., and Ohanessian, A. (1970).In Vitro 6:162.
Shields, G., and Sang, J.H. (1977).Drosophila Information Service 52:161.
Alahiotis, S., and Berger, E. (1977).Biochem. Genet. 15:877.
Alahiotis, S., and Berger, E. (1978).Comp. Biochem. Physiol. 61B:199.
Lowry, O.H., Rosenbrough, N.J., Farr, A.L., and Randall, R.J. (1951).J. Biol. Chem. 193:265.
Gehring, U., and Thompson, E.B. (1979). InGlucocorticoid Hormone Action, (eds.) Baxter, J.D., and Rousseau, G.G. (Springer-Verlag, Berlin, p. 399.
Dudai, Y. (1977).Drosophila Information Service 52:65.
Hall, J.C., and Kankel, D.R. (1976).Genetics 83:517.
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Berger, E., Wyss, C. Acetylcholinesterase induction byβ-ecdysone inDrosophila cell lines and their hybrids. Somat Cell Mol Genet 6, 631–640 (1980). https://doi.org/10.1007/BF01538642
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DOI: https://doi.org/10.1007/BF01538642