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Fluorescence analysis of late DNA replication in human metaphase chromosomes

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Somatic Cell Genetics

Abstract

Thymidine incorporated as a terminal pulse into chromosomes otherwise substituted with 5-bromodeoxyuridine can be detected by associated bright 33258 Hoechst fluorescence. The location of metaphase chromosome regions identified by this method as last to complete DNA synthesis is consistent with the results of autoradiographic analyses with tritiated thymidine. The very late-replicating regions correspond to a subset of those which appear as bands after chromosomes are stained by quinacrine or modified Giemsa techniques. The high resolution of the 33258 Hoechst fluorescence pattern within individual cells is especially useful for revealing variations in the order of terminal replication. Both homolog asynchrony and fluctuations in the distribution of bright 33258 Hoechst fluorescence within chromosomes from different cells are apparent and localized to individual bands. The results are consistent with the possibility that these bands constitute units of chromosome replication as well as structure.

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Literature cited

  1. Latt, S. A. (1973).Proc. Natl. Acad. Sci. U.S.A. 70:3395–3399.

    PubMed  Google Scholar 

  2. Caspersson, T., Zech, L., Johansson, C., and Modest, E. J. (1970).Chromosoma 30:215–217.

    PubMed  Google Scholar 

  3. Caspersson, T., Lindsten, J., Lomakka, G., Moller, A., and Zech, L. (1973).Int. Rev. Exp. Pathol. 11:1–72.

    Google Scholar 

  4. Miller, O. J., Miller, D. A., and Warburton, D. (1973).Prog. Med. Genet. 9:1–47.

    PubMed  Google Scholar 

  5. Hsu, T. C. (1973).Ann. Rev. Genet. 7:153–176.

    PubMed  Google Scholar 

  6. Latt, S. A. (1974).J. Histochem. Cytochem. 22:478–491.

    PubMed  Google Scholar 

  7. Latt, S. A. (1974).Exp. Cell Res. 86:412–415.

    PubMed  Google Scholar 

  8. Latt, S. A. (1974).J. Cell Biol. 62:546–550.

    PubMed  Google Scholar 

  9. Taylor, J. H. (1960).J. Biophys. Biochem. Cytol. 7:455–464.

    PubMed  Google Scholar 

  10. Lima-DeFaria, A., Reitalu, J., and Bergman, S. (1961).Hereditas 47:695–704.

    Google Scholar 

  11. Schmid, W. (1963).Cytogenetics 2:175–193.

    Google Scholar 

  12. German, J. L. (1964).J. Cell Biol. 20:37–55.

    PubMed  Google Scholar 

  13. Takagi, N., and Sandberg, A. A. (1968).Cytogenetics 7:118–134.

    PubMed  Google Scholar 

  14. Passarge, E., LaRock, J., and German, J. (1969).Chromosoma 28:37–47.

    PubMed  Google Scholar 

  15. Gianelli, F. (1970). Human chromosomes DNA synthesis. In Beckman, L. and Hauge, M. (eds.),Monographs in Human Genetics, Vol. 5, S. Karger, Basel, pp. 1–156.

    Google Scholar 

  16. Miller, O. J. (1970).Adv. Hum. Genet. 2:35–130.

    Google Scholar 

  17. Craig, A. P., and Shaw, M. W. (1971).Chromosoma 32:364–377.

    PubMed  Google Scholar 

  18. Rogers, A. W. (ed.) (1973).Techniques of Autoradiography, 2nd ed., Elsevier Press, Inc., Amsterdam.

    Google Scholar 

  19. Huberman, J. A., and Riggs, A. P. (1968).J. Mol. Biol. 32:327–341.

    PubMed  Google Scholar 

  20. Hand, R. (1975).J. Cell Biol. 64:89–97.

    PubMed  Google Scholar 

  21. Ganner, E., and Evans, H. J. (1971).Chromosoma 35:326–341.

    PubMed  Google Scholar 

  22. Breg, W. R., Allderdice, P. W., Miller, D. A., and Miller, O. J. (1972).Nature (London), New Biol. 236:76–78.

    Google Scholar 

  23. Calderon, D., and Schnedl, W. (1973).Humangenetik 18:63–70.

    PubMed  Google Scholar 

  24. Sasaki, M. S., and Norman, A. (1966).Nature 212:913–914.

    Google Scholar 

  25. Czaker, R. (1973).Humangenetik 19:135–144.

    PubMed  Google Scholar 

  26. McIlvaine, T. C. (1921).J. Biol. Chem. 49:183–186.

    Google Scholar 

  27. Latt, S. A. (1974).Science 185:74–76.

    PubMed  Google Scholar 

  28. Sumner, A. T., Evans, H. J., and Buckland, R. A. (1971).Nature (London), New Biol. 232:31–32.

    Google Scholar 

  29. Arrighi, F. E., and Hsu, T. C. (1971).Cytogenetics 10:81–86.

    PubMed  Google Scholar 

  30. Sumner, A. T. (1972).Exp. Cell Res. 75:304–306.

    PubMed  Google Scholar 

  31. Paris Conference 1971 (1972). Standardization in Human Cytogenetics.Cytogenetics 11:313–362.

    Google Scholar 

  32. Lin, M. S., Latt, S. A., and Davidson, R. L. (1974).Exp. Cell Res. 86:392–395.

    PubMed  Google Scholar 

  33. Latt, S. A., Lin, M. S., Davidson, R. L., and Gerald, P. S. (1974).Exp. Cell Res. 87:425–429.

    PubMed  Google Scholar 

  34. Djordjevic, B., and Szybalski, W. (1960).J. Exp. Med. 112:509–531.

    PubMed  Google Scholar 

  35. Hsu, T. C., and Somers, C. E. (1961).Proc. Natl. Acad. Sci. U.S.A. 47:396–403.

    PubMed  Google Scholar 

  36. Geraedts, J. P. M., and Pearson, P. L. (1974).Clin. Genet. 6:247–257.

    PubMed  Google Scholar 

  37. Slezinger, S. I., and Prokofieva-Belgovskaya, A. A. (1968).Cytogenetics 7:337–346.

    PubMed  Google Scholar 

  38. German, J., and Aronian, D. (1971).Chromosoma 35:99–110.

    PubMed  Google Scholar 

  39. Slezinger, S. I., Lozovskaya, E. R., Atayeva, D. M., and Prokofieva-Belgovskaya, A. A. (1975).Chromosoma 49:173–184.

    Google Scholar 

  40. Blumenthal, A. B., Kriegstein, H. J., and Hogness, D. S. (1973).Cold Spring Harbor Symp. Quant. Biol. 38:448–454.

    Google Scholar 

  41. Schnedl, W. (1971).Chromosoma 34:448–454.

    PubMed  Google Scholar 

  42. Unakul, W., Johnson, R. T., Rao, P. N., and Hsu, T. C. (1973).Nature (London) New Biol. 242:106–107.

    Google Scholar 

  43. Palmer, C. G. (1970).Can. J. Genet. Cytol. 12:816–830.

    PubMed  Google Scholar 

  44. Zakharov, A. F., and Egolina, N. A. (1972).Chromosoma 38:341–365.

    PubMed  Google Scholar 

  45. Baranovskaya, L. T., Zakharov, A. F., Dutrillaux, B., Capentier, S., Prieur, M., and Lejeune, J. (1972).Ann. Genet. (Paris) 15:271–274.

    Google Scholar 

  46. Baronovskaya, L. T., and Zakharov, A. F. (1974).Humangenetik 23:131–136.

    PubMed  Google Scholar 

  47. Dutrillaux, M. B., Laurent, C., Couturier, J., and Lejuene, J. (1973).C.R. Acad. Sci. Paris 276:3179–3181.

    Google Scholar 

  48. Lin, M. S., and Davidson, R. L. (1975).Somatic Cell Genet. 1:111–122.

    PubMed  Google Scholar 

  49. Marin, G., and Colletta, G. (1974).Exp. Cell Res. 89:368–376.

    PubMed  Google Scholar 

  50. Croce, C. M., Girardi, A. J., and Koprowski, H. (1973).Proc. Natl. Acad. Sci. U.S.A. 70:3617–3620.

    PubMed  Google Scholar 

  51. Schnedl, W. (1971).Humangenetik 20:55–58.

    Google Scholar 

  52. Chan, T. S., Long, C., and Green, H. (1973).Fed. Proc. 32:566a.

    Google Scholar 

  53. Chun, E., and Littlefield, J. W. (1961).J. Mol. Biol. 3:668–673.

    Google Scholar 

  54. Lin, S.-Y., and Riggs, A. P. (1974).Proc. Natl. Acad. Sci. U.S.A. 71:947–951.

    PubMed  Google Scholar 

  55. Weintraub, H. (1973).Cold Spring Harbor Symp. Quant. Biol. 38:247–256.

    Google Scholar 

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Authors and Affiliations

  1. Clinical Genetics Division, Children's Hospital Medical Center, USA

    Samuel A. Latt

  2. The Department of Pediatrics, Harvard Medical School, Boston, Massachusetts

    Samuel A. Latt

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  1. Samuel A. Latt
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Latt, S.A. Fluorescence analysis of late DNA replication in human metaphase chromosomes. Somat Cell Mol Genet 1, 293–321 (1975). https://doi.org/10.1007/BF01538452

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  • DOI: https://doi.org/10.1007/BF01538452

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