Abstract
The frequency of ethyl methanesulfonate (EMS)-induced mutations to 6-thioguanine resistance in a Chinese hamster ovary cells done K 1-BH4 was studied at many EMS doses including the minimally lethal range (0–100 μg/ml) as well as the exponential killing portion (100–800 μg/ml) of the survival curve. The mutation frequency increases approximately in proportion with increasing EMS concentration at a fixed treatment time. The pooled data for the observed mutation frequency, f(X), as a function of EMS dose X, is adequately described by a linear function f(X)=10−6(8.73+3.45 X), where 0≤X≤800 μg/ml. One interpretation of the linear dose-response is that, as a result of EMS treatment, ethylation of cellular constituents occurs, which is directly responsible for the mutation. Biochemical analyses demonstrate that most of the randomly isolated 6-thioguanine-resistant variants possess a highly reduced or undetectable level of HGPRT activity suggesting that the EMS-induced mutations to 6-thioguanine resistance affect primarily, if not exclusively, the HGPRT locus.
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Hsie, A.W., Brimer, P.A., Mitchell, T.J. et al. The dose-response relationship for ethyl methanesulfonate-induced mutations at the hypoxanthine-guanine phosphoribosyl transferase locus in Chinese hamster ovary cells. Somat Cell Mol Genet 1, 247–261 (1975). https://doi.org/10.1007/BF01538449
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DOI: https://doi.org/10.1007/BF01538449