Summary
Young healthy volunteers received a purine-free, isoenergetic formula diet over a period of 28 to 32 days. After a short time under formula diet alone 400 mg allopurinol were administered daily. After a further 10 days each volunteer received daily, in addition, either 4 g RNA, 4 g RNA-hydrolysate, 1 g guanosine-5-monophosphate (GMP), 1 or 3 g adenosine-5-monophosphate (AMP), uridine-5-monophosphate (UMP), cytidine-5-monophosphate (CMP) or adenosine, guanosine, uridine, cytidine, guanine, hypoxanthine, xanthine, cytosine and uracil. Finally the allopurinol was omitted. The renal excretion of total orotic acid (orotic acid and orotidine), uric acid and creatinine was determined daily; serum uric acid concentration and the enzyme activities of orotidine-5-phosphate-decarboxylase (ODCase) and hypoxanthine-guanine-phosphoribosyltransferase (HGPRTase) from erythrocytes were determined every other day. The results show that RNA, RNA-hydrolysate, purine- and pyrimidines and -nucleosides as well as hypoxanthine, and to a lesser extent adenine, diminish allopurinol-induced orotaciduria. This is compatible with an influence of dietary purines and pyrimidines on human pyrimidine biosynthesis.
Similar content being viewed by others
Literatur
Arnold WJ, Kelley WN (1971) Human Hypoxanthine-guanine-phosphoribosyltransferase: Purification and subunit structure. J Biol Chem 246:7398
Banholzer P, Gröbner W, Zöllner N (1980) Der Einfluß von Purinen sowie Oxipurinol auf den Pyrimidinstoffwechsel in menschlichen Lymphozytenkulturen. Verh Dtsch Ges Inn Med 86, S 928
Chen JJ, Jones ME (1979) Effect of 5-phosphoribosyl-1-pyrophosphate on de novo pyrimidine biosynthesis in cultured Ehrlich Ascites Cells made permeable with Dextran-Sulfate 500. J biol Chem 254:2697
Fox RM, Royse-Smith D, O'Sullivan WJ (1970) Orotidinuria induced by allopurinol. Science 168:861
Gröbner W, Kelley WN (1975) Effect of allopurinol and its metabolic derivatives on the configuration of human orotate phosphoribosyltransferase and orotidine-5-phosphate-decarboxylase. Biochem Pharmacol 24:379
Kelley WN, Beardmore TD (1970) Allopurinol: Alteration in pyrimidine metabolism in man. Science 169:388
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the folin phenol reagent. J Biol Chem 193:165
Rogers LE, Porter FSt (1968) Hereditary orotic aciduria II. A urinary screening test. Pediatrics 42:423
Steudel H, Peiser E (1922) Über die Hefenucleinsäure. Z Phys Chem 120:292
Zöllner N (1963) Eine einfache Modifikation der enzymatischen Harnsäurebestimmung. Normwerte in der deutschen Bevölkerung. Z Klin Chem 1:178
Zöllner N, Gröbner W (1971) Influence of oral ribonucleic acid on orotaciduria due to allopurinol administration. Z Ges Exp Med 156:317
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Gröbner, W., Zöllner, N. Der Einfluß von Nahrungspurinen und -pyrimidinen auf die Pyrimidinsynthese des Menschen. Klin Wochenschr 61, 1191–1197 (1983). https://doi.org/10.1007/BF01537430
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01537430