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Effect of sucralfate and its components on taurocholate-induced damage to rat gastric mucosal cells in tissue culture

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Abstract

The present study evaluated the effect of sucralfate and its components, sucrose octasulfate and aluminum hydroxide, on: (1) damage to rat cultured gastric mucosal cells induced by sodium taurocholate in a neutral environment and in conditions independent of systemic factors, (2) prostaglandin E2 and on 6-keto prostaglandinF 1α release by cultured cells, and (3) sulfhydryl content of cultured cells. Cell damage was quantitated by chromium-51 release assay. Prostaglandin E2 and 6-keto prostaglandinF 1α were measured by radioimmunoassay. Total sulfhydryl content of cultured cells was determined calorimetrically. Microscopically, sucralfate was found to adhere tightly to epithelial cell surfaces despite frequent washings. Sucralfate 2 mg/ml and 5 mg/ml significantly decreased taurocholate-induced damage, reducing taurocholate-induced specific51Cr release by 11.8 points (equal to 29% decrease in cell damage, (P< 0.01) and 22.9 points (equal to 56% decrease in cell damage, (P<0.001), respectively. Sucrose octasulfate and aluminum hydroxide did not exert significant protection against damage induced by sodium taurocholate. The protective effect of sucralfate was not prevented by indomethacin, nor was it counteracted by the sulfhydryl blocker, iodoacetamide. Sucralfate, but not its components, significantly and dose-dependently stimulated prostaglandin E2 (r=0.94, P<0.05 and 6-keto prostaglandinF 1α (r=0.89, P<0.05) production by cultured cells. Neither sucralfate nor its components affected sulfhydryl content of cultured cells. In conclusion, sucralfate, but not its components, (1) protects rat gastric mucosal cells against taurocholate-induced damage in conditions independent of systemic factors and in a neutral environment and (2) significantly stimulates prostaglandin production by cultured cells. (3) The protection by sucralfatein vitro does not seem to depend on its stimulatory effect on endogenous prostaglandin synthesis.

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Authors and Affiliations

  1. Department of Medicine, Long Beach VA Medical Center and University of California, Irvine, California

    Marco Romano MD, Mahnaz Razandi MS & Kevin J. Ivey MD

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  1. Marco Romano MD
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  2. Mahnaz Razandi MS
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  3. Kevin J. Ivey MD
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Dr. Romano was a visiting scientist from Istituto di Medicina Generale e Metodologia clinica, 1st School of Medicine, University of Naples, Italy.

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Romano, M., Razandi, M. & Ivey, K.J. Effect of sucralfate and its components on taurocholate-induced damage to rat gastric mucosal cells in tissue culture. Digest Dis Sci 35, 467–476 (1990). https://doi.org/10.1007/BF01536921

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  • DOI: https://doi.org/10.1007/BF01536921

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