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Discrepancies between gastric mucosal and urinary pepsinogen A patterns andin vitro synthesis and secretion of human pepsinogen

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Abstract

The relationship between electrophoretic pepsinogen A (PGA) patterns from urine and gastric mucosa was studied in healthy volunteers and in patients with various gastric disorders. Discrepancies between urinary and gastric PGA patterns were found in 63.3% of the individuals. In 9% of the subjects with these discrepancies, the phenotype class in urine was different from that in gastric mucosa. The differences were found in all diagnostic groups. The highest frequency of differences was found in patients with gastric ulcer. The differences were not related to the serum PGA level. More than 80% of the differences were caused by a lower relative intensity of pepsinogen A fraction 5 (Pg5) in urine than in gastric mucosa. The possible origin of differences in PGA isozymogen patterns was studied by organ culture of gastric biopsies. In vitro synthesis and secretion of pepsinogens were studied by electrophoresis and autoradiography. The synthesis rate of PGA in biopsies of 1–2 mm diameter was 40–100 ng/hr. Posttranslational modification of PGA isozymogens was demonstrated. Pg2 and part of Pg4 probably are secondary products of Pg3 and Pg5, respectively. In some individuals the secretion rate ofPg3 was low compared to the other isozymogens. The conversion of Pg3 into Pg2 and the differential secretion of the isozymogens may explain some of the discrepancies between gastric and urinary PGA patterns.

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Authors and Affiliations

  1. Institute of Human Genetics and Department of Gastroenterology, Free University, Amsterdam, The Netherlands

    Gerard Pals PhD, Barend D. Westerveld PhD, Jacques Defize PhD, Jan C. Pronk PhD, Henk Brand MSc, Marja Flipse MSc, Cor Verwey MSc, Stephan G. M. Meuwissen PhD & Aldur W. Eriksson PhD

Authors
  1. Gerard Pals PhD
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  2. Barend D. Westerveld PhD
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  3. Jacques Defize PhD
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  4. Jan C. Pronk PhD
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  5. Henk Brand MSc
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  6. Marja Flipse MSc
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  7. Cor Verwey MSc
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  8. Stephan G. M. Meuwissen PhD
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  9. Aldur W. Eriksson PhD
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Additional information

The incorporation studies were carried out at the Radio Nuclide Centre of the Free University.

This study was supported by grant VUGE-81 of the Koningin Wilhelmina Fonds.

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Pals, G., Westerveld, B.D., Defize, J. et al. Discrepancies between gastric mucosal and urinary pepsinogen A patterns andin vitro synthesis and secretion of human pepsinogen. Digest Dis Sci 33, 135–143 (1988). https://doi.org/10.1007/BF01535723

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  • DOI: https://doi.org/10.1007/BF01535723

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