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Retinoic acid modulation of mucin mRNA in rat tracheal explants: Response to actinomycin D, cycloheximide, signal transduction effectors and antisense oligodeoxynucleotide

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Abstract

Factors affecting the retinoic acid modulated expression of mucin mRNA in rat tracheal cultures were studied. Actinomycin D had no effect on mucin mRNA in cultures grown with retinoic acid (RA+). The usual precipitous drop in mucin mRNA in cultures lacking retinoic acid (RA−) was prevented by actinomycin D. Cycloheximide also had no effect on mucin mRNA in RA+ cultures, but, like actinomycin D, it prevented the precipitous drop in mucin mRNA in RA− cultures. cAMP agonists had some marginal effects on the mucin mRNA, but none as dramatic as those noted by actinomycin D and cycloheximide in the RA− cultures. An antisense oligomer (18 bases) to rat mucin cDNA inhibited the mucin mRNA expression in RA+ cultures.

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Authors and Affiliations

  1. Department of Clinical Investigation, William Beaumont Army Medical Center, 79920, El Paso, Texas

    Sambhu N. Bhattacharyya & Brigitta Manna

  2. Electronmicroscopy Section, William Beaumont Army Medical Center, 79920, El Paso, Texas

    Patrick Ashbaugh

  3. Department of Biochemistry, Duke University Medical Center, 27710, Durham, North Carolina

    Bernard Kaufman

Authors
  1. Sambhu N. Bhattacharyya
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  2. Patrick Ashbaugh
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  3. Bernard Kaufman
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  4. Brigitta Manna
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The opinions or assertions contained herein are the private views of the authors and are not to be construed as official or as reflecting the views of the Department of the Army or the Department of Defense.

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Bhattacharyya, S.N., Ashbaugh, P., Kaufman, B. et al. Retinoic acid modulation of mucin mRNA in rat tracheal explants: Response to actinomycin D, cycloheximide, signal transduction effectors and antisense oligodeoxynucleotide. Inflammation 18, 565–574 (1994). https://doi.org/10.1007/BF01535255

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