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Synergistic enzyme induction by glucocorticoids and cyclic AMP observed in glioma x hepatoma cell hybrids but not in their parents

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Somatic Cell and Molecular Genetics

Abstract

Enzyme induction by hydrocortisone (HC) and dibutyryl cyclic AMP (dbcAMP) was studied in C6 rat glioma cells, FU5AH rat hepatoma cells, and five C6 x FU5AH hybrids. Hormone responsive enzymes from both parental lines were studied, including: tyrosine aminotransferase (TAT), alanine aminotransferase (AAT), glycerol phosphate dehydrogenase (GPDH), lactate dehydrogenase (LDH), and 2′,3′-cyclic nucleotide 3′-phosphohydrolase (CNP). There was no overall dominance of one parental phenotype over the other in expression of uninduced or induced enzyme activity after fusion, and the hybrids possessed some enzymatic properties characteristic of both parents. GPDH was induced by dbcAMP in all five hybrids, and TAT was induced by dbcAMP in four of the hybrids, although neither of these enzymes were induced by dbcAMP in the parents. Furthermore, synergistic induction of these enzymes by HC and dbcAMP was observed in the hybrids but not in the parents. These hybrids provide a model system to study hormone interaction in enzyme induction.

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  1. Robert D. Meyer

    Present address: Chestnut Hill College, 19118, Philadelphia, Pennsylvania

Authors and Affiliations

  1. The Wistar Institute of Anatomy and Biology, 19104, Philadelphia, Pennsylvania

    Robert D. Meyer & F. Arthur McMorris

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Meyer, R.D., McMorris, F.A. Synergistic enzyme induction by glucocorticoids and cyclic AMP observed in glioma x hepatoma cell hybrids but not in their parents. Somat Cell Mol Genet 10, 153–159 (1984). https://doi.org/10.1007/BF01534904

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  • DOI: https://doi.org/10.1007/BF01534904

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