Somatic Cell and Molecular Genetics

, Volume 15, Issue 3, pp 229–235 | Cite as

Long-range structure of H-ras 1-selected transgenomes

  • Wendy A. Bickmore
  • John C. Maule
  • Veronica van Heyningen
  • David J. Porteous


We have used chromosome-mediated gene transfer (CMGT) and whole cell fusion to derive human-mouse hybrid cells carrying reduced human chromosomes 11, by selecting for expression of the transforming H-ras 1 oncogene. To realize the full potential of these somatic cell genetic techniques as resources for enriched DNA probe isolation and the fine structure mapping of chromosomes, the nature of any molecular rearrangements that may accompany the process of DNA transfer must be understood. We have analyzed the long-range structure of our transgenomes by pulsed field gel electrophoresis (PFGE) and show here that, whereas during cell fusion several megabase pairs (Mb) of DNA can be transferred intact, multiple rearrangements of DNA accompany CMGT even in transgenomes where other methods of analysis gave no indication of such molecular scrambling.


Somatic Cell Structure Mapping Human Chromosome Full Potential Cell Fusion 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Literature cited

  1. 1.
    Porteous, D.J. (1987).Trends Genet. 3:177–182.Google Scholar
  2. 2.
    Porteous, D.J., Morten, J.E.N., Cranston, G., Fletcher, J.M., Mitchell, A., van Heyningen, V., Fantes, J.A., Boyd, P.A., and Hastie, N.D. (1986).Mol. Cell. Biol. 6:2223–2232.PubMedGoogle Scholar
  3. 3.
    Gosden, J.R., and Porteous, D.J. (1987).Cytogenet. Cell Genet. 45:44–51.PubMedGoogle Scholar
  4. 4.
    Porteous, D.J., Bickmore, W., Christie, S., Boyd, P.A., Cranston, G., Fletcher, J.M., Gosden, J.R., Rout, D., Seawright, A., Simola, K.O.J., van Heyningen, V., and Hastie, N.D. (1987).Proc. Natl. Acad. Sci. U.S.A. 84:5355–5359.PubMedGoogle Scholar
  5. 5.
    Bickmore, W., Christie, S., van Heyningen, V., Hastie, N.D., and Porteous, D.J. (1988).Nucleic Acids Res. 16:51–60.PubMedGoogle Scholar
  6. 6.
    Porteous, D.J., Morten, J.E.N., Forster, M.E., Cranston, G., Weir-Thompson, E., Busuttil, A., Bostock, C., and Steel, C.M. (1986).Int. J. Cancer 38:603–612.PubMedGoogle Scholar
  7. 7.
    Southern, E.M., Anand, R., Brown, W.R.A., and Fletcher, D.S. (1987).Nucleic Acids Res. 15:5925–5943.PubMedGoogle Scholar
  8. 8.
    Feinberg, A.P., and Vogelstein, B. (1984).Anal. Biochem. 137:266–267.PubMedGoogle Scholar
  9. 9.
    Church, G.M., and Gilbert, W. (1984).Proc. Natl. Acad. Sci. U.S.A. 81:1991–1995.PubMedGoogle Scholar
  10. 10.
    Watkins, P.C., Eddy, R., Beck, A.K., Velluci, V., Leverone, B., Tanzi, R.E., Gusella, J.F., and Shows, T.B. (1987).DNA 6:205–212.PubMedGoogle Scholar
  11. 11.
    Miller, C.L., and Ruddle, F.H. (1978).Proc. Natl. Acad. Sci. U.S.A. 75:3346–3350.PubMedGoogle Scholar
  12. 12.
    Klobutcher, L.A., and Ruddle, F.H. (1979).Nature 280:657–660.PubMedGoogle Scholar
  13. 13.
    Pritchard, C.A., and Goodfellow, P.N. (1987).Genes and Development 1:172–178.PubMedGoogle Scholar
  14. 14.
    Wullems, G.J., van der Horst, J., and Bootsma, D. (1976).Somat. Cell Genet. 3:359–371.Google Scholar
  15. 15.
    Simmons, T., Lipman, M., and Hodge, L.D. (1978).Somat. Cell Genet. 4:55–76.PubMedGoogle Scholar
  16. 16.
    Harris, H. (1970). Cell Fusion. (Clarendon Press, London).Google Scholar
  17. 17.
    Bostock, C.J., and Allshire, R.C. (1986).Somat. Cell Mol. Genet. 12:357–366.PubMedGoogle Scholar

Copyright information

© Plenum Publishing Corporation 1989

Authors and Affiliations

  • Wendy A. Bickmore
    • 1
  • John C. Maule
    • 1
  • Veronica van Heyningen
    • 1
  • David J. Porteous
    • 1
  1. 1.MRC Human Genetics UnitWestern General HospitalEdinburghScotland

Personalised recommendations