Abstract
Mitochondria from two different cytoplasmically inherited oligomycin-resistant Chinese hamster ovary cell lines synthesize an altered polypeptide compared to mitochondria from wild-type cells. For example, mitochondria from both oligomycin-resistant cell lines synthesize a polypeptide with a molecular weight of approximately 20,500, which is present in very low amounts in wild-type cells. In contrast, mitochondria from wild-type cells synthesize a polypeptide with a molecular weight of approximately 19,500, which is present in very low amounts in one of the oligomycin-resistant mutants and in reduced amounts in the other mutant. The gene which encodes this altered polypeptide is cytoplasmically transferred together with the oligomycin-resistant phenotype. This is the first example in mammalian cells where an altered mitochondrial gene product is shown to be associated with the cytoplasmic transfer of oligomycin resistance.
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Shew, J.Y., Breen, G.A.M. Two cytoplasmically inherited oligomycin-resistant Chinese hamster cell lines exhibit an altered mitochondrial translation product. Somat Cell Mol Genet 11, 103–108 (1985). https://doi.org/10.1007/BF01534741
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DOI: https://doi.org/10.1007/BF01534741