Abstract
Embryos of 21 temperature-sensitive Drosophilamutants were used to set up 232 primary cultures. From these, 57 cell lines are being subcultured, and we consider 33 of them to be continuous. Nineteen of the 21 mutants are represented in these 57 lines. Four characteristics of each of the continuous lines are described: morphology, ecdysterone response, karyotype, and response to a restrictive temperature. We discuss the potential uses of such cell lines.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Literature cited
Kakpakov, V.T., Gvozdev, V.A., Platova, T.P., and Polukalova, L.G. (1969).Genetika 5:67–75; English translation:Soviet Genet. 5:1647–1655.
Echalier, G., and Ohanessian, A. (1969).C.R. Acad. Sci. (Paris) D 268:1771–1773.
Echalier, G., and Ohanessian, A. (1970).In Vitro 6:162–172.
Courgeon, A.M. (1972).Exp. Cell Res. 74:327–336.
Schneider, I. (1972).J. Embryol. Exp. Morphol. 27:353–365.
Berger, E.M., Frank, M., and Abell, M.C. (1980). InInvertebrate Systems in Vitro, (eds.) Kurstak, K., Maramorosch, K., and Dubendorfer, A. (Elsevier/North-Holland Biomedical Press, New York), pp. 195–208.
Cherbas, L., Cherbas, P., Savakis, C., Demetri, G., Manteuffel-Cymborowska, M.D., Yonge, C.F., and Williams, C. (1980). InInvertebrate Systems in Vitro, (eds.) Kurstak, K. Maramorosch, K., and Dubendorfer, A. (Elsevier/North-Holland Biomedical Press, New York), pp. 217–228.
Schlesinger, M.J., Ashburner, M., and Tissieres, A. (1982).Heat Shock from Bacteria to Man (Cold Spring Harbor Laboratory, Cold Spring Harbor, New York).
Schneider, I., and Blumenthal, A.B. (1978). InThe Genetics and Biology of Drosophila, Vol 2a, (eds.) Ashburner, M., and Wright, T.R.F. (Academic Press, New York), pp. 265–315.
Hink, W.F. (1980). InInvertebrate Systems in Vitro, (eds.) Kurstak, K., Maramorosch, K., and Dubendorfer, A. (Elsevier/North-Holland Biomedical Press, New York), pp. 553–578.
Regenass, U., and Bernhard, H.P. (1979).Wilhelm Roux. Arch. 187:167–177.
Lindsley, D.L., and Grell, E.H. (1968).Genetic Variations of Drosophila Melanogaster. Carnegie Institute, Washington, Publ. No. 627.
Shields, G., and Sang, J.H. (1977).Drosophila Information Service.52:161.
Arking, R. (1974).Genetics 80:519–539.
Fattig, W.D., and Rickoll, W.L. (1979).Genetics 71:309–313.
Shields, G., Dubendorfer, A., and Sang, J.H. (1975).J. Embryol. Exp. Morphol. 33:159–175.
Shields, G., and Sang, J.H. (1970).J. Embryol. Exp. Morphol. 23:53–65.
Rosset, R. (1978).Exp. Cell Res. 111:31–36.
Stevens, B., Alvarez, L.M., Bohman, R., and O'Connor, J.D. (1980).Cell 22:675–682.
Maroy, P., Dennis, R., Beckers, C., Sage, B.A., and O'Connor, J.D. (1978).Proc. Natl. Acad. Sci. U.S.A. 75:6035–6038.
Stevens, B., and O'Connor, J.D. (1982).Dev. Biol. 94:176–182.
Lowy, I., Pellicer, A., Jackson, J.F., Sim, G.K., Silverstein, S., and Axel, R. (1980).Cell 22:817–823.
Perucho, M., Hanahan, D., Lipsich, L., and Wigler, M. (1980).Nature 285:207–210.
Sang, J.H. (1981). InAdvances in Cell Culture, Vol. 1, (ed.) Maramorosch, K. (Academic Press, New York), pp. 125–182.
Simcox, A.A., Cheney, C.M., and Shearn, A. Submitted.
Sang, J.H., Sinclair, J.M., Burke, J.F., and Ish-Horowicz, D.Somat. Cell Mol. Genet. (in press).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Simcox, A.A., Sobeih, M.M. & Shearn, A. Establishment and characterization of continuous cell lines derived from temperature-sensitive mutants ofDrosophila melanogaster . Somat Cell Mol Genet 11, 63–70 (1985). https://doi.org/10.1007/BF01534735
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF01534735