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High-frequency deletion event ataprt locus of CHO cells: Detection and characterization of endpoints

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Somatic Cell and Molecular Genetics

Abstract

Two mechanisms are implicated in generating recessive drug resistance mutants at the adenine phosphoribosyltransferase (aprt) locus of Chinese hamster ovary (CHO) cells, one of which is a spontaneous high-frequency deletion of the entire gene. We have isolated and mapped a 19-kb fragment carrying aprtand its flanking sequences. A Southern blot study of 198 independent deletion mutants revealed that two different mutants have one of their breakpoints within the 19-kb region analyzed. One of these has an upstream breakpoint which could be narrowed down to a 4-b fragment containing repetitive sequences. The other mutant has a breakpoint within a 410-bp sequence located 8.5 kb downstream of the aprtgene and which carries several elements similar to those signaling V-(D) -J joining in immunoglobulin and T-cell receptor gene rearrangements. In each case the other breakpoint lay outside of the analyzed region. These results support the previous indications that the deletions created by this spontaneous event are large.

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  1. Institut du Cancer de Montréal, 560, rue Sherbrooke Est, H2L4M1, Montréal, Québec, Canada

    Pascale Dewyse & W. E. C. Bradley

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  1. Pascale Dewyse
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  2. W. E. C. Bradley
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Dewyse, P., Bradley, W.E.C. High-frequency deletion event ataprt locus of CHO cells: Detection and characterization of endpoints. Somat Cell Mol Genet 15, 19–28 (1989). https://doi.org/10.1007/BF01534666

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  • DOI: https://doi.org/10.1007/BF01534666

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